Zoology-Master Dissertation
http://kr.cup.edu.in/handle/32116/1771
2024-03-28T23:50:14ZTo Study the Anti-Proliferative Effect of Pirfenidone via MAP Kinase Signaling Pathway
http://kr.cup.edu.in/handle/32116/1794
To Study the Anti-Proliferative Effect of Pirfenidone via MAP Kinase Signaling Pathway
Bharti, Pooja
Pirfenidone is a pyridine chemical compound used as a therapeutic drug used for the treatment of Idiopathic Pulmonary Fibrosis (IPF). The characteristic feature of the fibrosis is the deposition of extracellular matrix protein in the lungs and ultimate lead to the failure of the organ. Pirfenidone is an orally active small molecular comprising a modified phenyl pyridine that have been shown to inhibit the progression of fibrosis in animal model and in patient with IPF. The compound exhibits well?documented anti-fibrotic and anti? inflammatory activities but its molecular target has not been elucidated. Pirfenidone is a broad-spectrum anti-fibrotic drug with the ability to decreases the PDGF, TGF-?, TNF-? and COL (collagen). After treating A549 cell line with Pirfenidone with different concentrations and different time interval, cell viability was measured by the MTT assay. Pirfenidone cause the cytotoxicity in model A549 cell line and anti-proliferation through different Smad dependent and independent pathway. Pirfenidone treatment was given to check downstream signaling pathway i.e. MAP kinase activity dependent. Although previous studies have shown that Pirfenidone is associated with renal fibrosis in TGF??1 expression the mechanism remains poorly understood. Taken into account these, we hypothesized that pirfenidone may have an anti?fibrotic effect through antagonizing the MAPK signaling pathway.
2018-01-01T00:00:00ZTo check the glioprotective effect of Withaferin A on C6 Glioma cell Culture challenged with Kainic Acid
http://kr.cup.edu.in/handle/32116/1795
To check the glioprotective effect of Withaferin A on C6 Glioma cell Culture challenged with Kainic Acid
Dash, Bindu Balaya
Last many years perception of glial cell function in CNS has been changed. In fact abnormalities in glial cells also contribute to various disorders. Glutamate induced excitotoxicity attributed to various CNS related diseases. Kainic acid is a potent agonist of kainate receptors, a subclass of glutamate receptors, is 30 fold more neurotoxic than glutamate. Natural herbal extracts are attracting researchers for their pharmacologic properties against diseases associated with CNS. Root and leaf extracts of Withania somnifera, used since many years to treat several diseases in traditional medicine system. Present study was designed to see the glioprotective potential of Withaferin A, a natural extract from Withania somnifera or Ashwagandha, against Kainic acid induced excitotoxicity in C6 glioma cell line. Pretreatment of 0.5 µM Withaferin A showed defensive potential against 100 µM and 200 µM concentration of Kainic acid. To check expression of GFAP (well known marker of astrocytes) and NCAM, Immunocytochemistry was performed. Withaferin A treatment helps in normalizing of GFAP (Glial Fibrillary Acidic Protein) and NCAM (Neural Cell Adhesion Molecule) expression in kainic acid exposed cells. Our result suggested that Withaferin A have defensive potential against kainic acid induced excitotoxicity. As a potent glioprotective agent, Withaferin A could be used as therapeutic drug to treat glioblastomas and other neurological disorders.
2018-01-01T00:00:00ZNeuro-Protective Role Of Ginkgolide B In A?induced Neurodegeneration And Ache Enzyme Activity In Human Neuroblastoma Sh-Sy5Y Cells
http://kr.cup.edu.in/handle/32116/1796
Neuro-Protective Role Of Ginkgolide B In A?induced Neurodegeneration And Ache Enzyme Activity In Human Neuroblastoma Sh-Sy5Y Cells
Mukherjee, Ankita
Ginkgolide B (GB) is being used as medicine in China for treating neurodegenerative diseases for a long time. Its neuroprotective role is getting well established. Alzheimer's disease (AD) is a neurodegenerative disease that has multiple factors associated with its onsetand is one of the most common causes of dementia in the world. GB is known to reduce the oxidative stress caused due to accumulation of amyloid beta (A?), a major hallmark of AD associated strongly with the production of oxidative stress via production of ROS. The increase in the expression of AChE has been reported and it has been associated with increased toxicity of A?. This study tried to decipher the relationship between A?, GB and AChE activity. In this study, it was found that A?(25-35)-induced oxidative stress leads to increased production of ROS and decreased AChE activity. On the other hand, GB decreased ROS production and expression of AChE, thus pointing toward its protective effect. GB increased the activity of AChE, suggesting that due to its antioxidant potentialit possibly caused a decrease in protein oxidation, and thus increased the activity of the AChE enzyme. Therefore, the results of the present study show the modulatory role of GB an AChE enzyme activity under oxidative stress conditions as seen in AD, suggesting the potential of GB in AD therapeutics
2018-01-01T00:00:00ZExpression analysis of long non-coding RNA GAS5 and BANCR in lung cancer cell line A549 compared to IMR-90.
http://kr.cup.edu.in/handle/32116/1801
Expression analysis of long non-coding RNA GAS5 and BANCR in lung cancer cell line A549 compared to IMR-90.
Sharma, Uttam
Lung cancer is the major cause of death worldwide. Several chemotherapeutic drugs and therapies have been established, but the early diagnosis and prognosis of lung cancer is still a question. Long non-coding RNAs are important regulator molecules in the human genome, which serves as transcriptional modulator, post transcriptional processor, chromatin remodeletor and splicing regulator during the gene modification process. Emerging studies have suggested the role of long noncoding RNA as potential biomarker for cancer diagnosis and prognosis by functioning as tumor suppressors and oncogenes. Several studies have been reported on cell lines, tissues as well as tumor and the molecular mechanism is still not clearly understood. GAS5 and BANCR are two long non-coding RNAs, which are found to be down-regulated in multiple cancers such as lung carcinoma, breast cancer, etc. In the current study, we focus on the expression analysis of GAS5 and BANCR in A549 cell line compared to IMR-90 cell line to study the role of long non-coding RNA in the pathogenesis of lung cancer. Furthermore, we investigated the expression of GAS5 and BANCR using quantitative Real-Time PCR. The result showed that GAS5 and BANCR expression was significantly down-regulated in cancerous cell line compared to non-cancerous cell line. The fold change of lncRNAs GAS5 and BANCR was 14 times (P=0.0088) and 7 times (P=0.0088) down-regulated in A549 cell line respectively. The melt curve analysis showed that there was only one sharp peak obtained for both GAS5 and BANCR i iv which suggests that primers bind to their specific targets and no primer dimer was observed.
2018-01-01T00:00:00Z