The prime objective of the present work is to isolate, characterize and purify soil microbes and to know about their antibacterial and anticancer properties. Soil samples were obtained from different locations in Dharamsala, Himachal Pradesh, India. Serial dilutions of the samples were made and plated on starch casein agar. Screening, purification and further tests were performed on the isolates to find the potential colonies. Biochemical and morphological characterization was done as well. Following characterization, isolates were used to extract bioactive secondary metabolites which can be anti-bacterial and anti-cancer. Secondary screening for antibacterial properties of isolates was evaluated by Agar well diffusion method. Further, the isolates were subjected to the GC-MS and MTT cytotoxic assay. Totally 7 isolates of actinomycetes were isolated and out of them only 4 were tested for antagonistic activity against 4 pathogenic microorganisms. Isolates M1,and M3 of dilution 10-1 were active, while M2 and M4 showed less activity against the pathogenic microorganisms. All actinomycetes isolates showed antibacterial activity against S. enterica and P. putida while they showed less activity against S. aureus and E.coli. These isolates showed antibacterial and anti-cancer activities and may be used for the growth of new antibiotics for pharmaceutical or agricultural purposes.