The studies area is lies along the western most part of the Indian sub-continent between, the latitude of 23.13°-24.68° N and longitude 68.10°-71.80° E, which is pre cratonic, is preserving almost a complete sequence of sedimentary deposits ranging in age from Triassic to Recent. This dissertation attempts to study the microstructural analysis of tooth’s enamel of mammals of dinotherium, gompotherium, hipparion, rhino from Miocene deposit of Kutch basin Dental materials of deinotherium, gompotherium, hipparion, rhino of Kutch basin was collected and sampled for comprehensive analysis of the microstructure of the enamel. Microstructural features of the mammalian tooth enamel used for taxonomical identification of the analysed sample. I investigated the deinotheirum, Gompotheriidae, Rhinoceritidae, hipparion. Microstructures can be analysed by the stacking pattern of the prisms, different species belonging to different family shows different types of stacking pattern called HSB. The results obtained from study of the SEM images suggest that different species of different family show different enamel prism pattern. Apart from taxonomical identification of the studied sample one can also find out dietary habits, palaeo-environment and how substances with high hardness can be chewed and grinded by enamel which is of less hardness.