| dc.contributor.author | Saxena, Aditi | |
| dc.date.accessioned | 2018-08-31T04:13:49Z | |
| dc.date.available | 2018-08-31T04:13:49Z | |
| dc.date.issued | 2018 | |
| dc.identifier.citation | Saxena, Aditi (2018) Chemical Investigation and Antiproliferative screening of extracts from Stevia rebaudiana (Bertoni) | en_US |
| dc.identifier.uri | http://kr.cup.edu.in/handle/32116/1938 | |
| dc.description.abstract | Cancer is the uncontrolled development of abnormal cells in the body. It is considered
as the leading public health problem in both developed and the developing countries. As
no drug of cancer is establish to be completely efficient and safe as anticancer therapy
and is responsible for the prolonged toxicity and also causes various side effects.
Chemoprevention of cancer by natural products is beneficial, as these compounds have
the nominal side effects and short of toxicity compared to the synthetic compounds. The
phenomena of Carcinogenesis is very complex and includes so many signaling
pathways. Thus Phytochemicals are measured as the right candidates for developing
the anticancer drug. The study for developing more potent candidates which can
obstruct or slow down the expansion of the cancer cells without c au s in g any side
effects from these phytochemicals are still in progress and Many new phytochemicals
and its derived analogs have been recognized as potential candidates for anticancer
therapy among these one of the potent plant is Stevia rebaudiana. The leaves of Stevia
rebaudiana tends to possess zero calories, and consists mainly of ent kaurene
diterpene glycosides generally recognized as steviol glycosides. Stevioside is the main
sweet component found in S. rebaudiana. studies suggest that the stevioside along with
v
other associated compounds including rebaudioside A, steviol, and isosteviol tends to
have therapeutic benefits including anticancer activity. Taking in consideration the
above mentioned factors we have investigated the Anticancer potential of extracts of
S. rebaudiana. Four extracts was prepared using petroleum ether, chloroform, ethyl
acetate and aqueous methanol. T47D cell line have been used to evaluate the
anticancer potential using MTT assay. AD-2 that is chloroform extract showing IC50
value of 7.79μg/ml. Moreover IC50 value of AD-4 that is aqueous methanol was also
comparatively better and found to be 9.53 μg/ml and AD-1 that is petroleum ether had
shown IC50 value of 9.58μg/ml. Thus, various extracts have shown good Antiproliferative
activity and S. rebaudiana can be further investigated for its anticancer potential.
Furthermore docking study on estrogen receptor–alpha, androgen receptor and
aromatase receptor discovered that the phytochemicals of the plant have good binding
affinity towards all the three mentioned receptors and can be suitably customized to
search its anticancer potential. Moreover unfavorable ADME profile can be overcome by
structure modification. Thus on the basis of in-vitro and in-silico data we can conclude
that S. rebaudiana extracts have promising anticancer potential. further isolation of
compounds have been done successfully and total four compounds have been isolated.
two compounds ASP-2 and ASP-4 have been successfully characterized and found to
be Stevioside and Rebaudioside A respectively which are already known compounds. | en_US |
| dc.language.iso | en_US | en_US |
| dc.publisher | Central University of Punjab | en_US |
| dc.subject | Stevia rebaudiana | en_US |
| dc.subject | SGs | en_US |
| dc.subject | Isolation | en_US |
| dc.subject | Antiproliferative | en_US |
| dc.subject | Receptors | en_US |
| dc.title | Chemical Investigation and Antiproliferative screening of extracts from Stevia rebaudiana (Bertoni) | en_US |
| dc.type | Master Dissertation | en_US |
| dc.contributor.supervisor | Jaitak, Vikas | |
| dc.identifier.accessionno | T00749 | |
