Pharmaceutical Sciences and Natural Products - Master Dissertation

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Browsing Pharmaceutical Sciences and Natural Products - Master Dissertation by Author "Gupta, Vinay Kumar"

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    In-vitro guided fractionation of crude root extracts of potentilla atrosanguinea lodd and in-silico of polyphenolic compounds
    (Central University of Punjab, 2013) Gupta, Vinay Kumar; Jaitak, Vikas
    Modern therapeutic system is a kind of inspiration from traditional plant based medicine used for various diseases and ailments. Advancement in drug discovery technology including computational drug design and bioassay guided fractionation emboldens the interest of Medicinal Chemists? concerning to lead identification from medicinal plants for complicated diseases, in the last few years. There are so many traditional plants which are used in the treatment of various diseases in different parts of our country but scientific information is missing for the same. Potentilla atrosanguinea is a native to the western Himalaya region has been used traditionally for the treatment of wound-healing, diarrhea, influenza and bleeding but there is not even a single published evidence about its activity accept antioxidant activity of aerial part. In this context, the aim of the present study was to explore the roots of P.atrosanguinea in terms of its medicinal value for instances in-vitro photoprotective and antioxidant activity. The photoprotective activity was evaluated in the term of SPF (sun protection factor) by spectrophotometric method in the range of 290-320 nm (UVB region) whereas antioxidative activity was evaluated using a free radical scavenging assay (DPPH, superoxide anion scavenging and CUPRAC). Total phenolic contents of the extract/fractions were v determined by Folin Ciocalteu reagent. The ability of photo-protection of different fraction against UVB region followed the trend Pa-AcOEt > Pa- n-BuOH > Pa-H2O-MeOH > Pa-H2O. Ethyl acetate fraction of Potentilla atrosanguinea indicated the highest sun protection factor (SPF) (7.319 ' 0.353) at a concentration of 120 ?g/ml. IC50 values of aqueous methanolic (Pa-H2O-MeOH) and ethyl acetate fraction (Pa-AcOEt) for DPPH assay was comparable as that of rutin (80 ?g/ml). Superoxide anion scavenging activity of all fractions was found to be excellent than standard (IC50 150 ?g/ml). Calculated IC50 value for the aqueous-methanolic, ethyl acetate, n-butanol (Pa- n-BuOH) and aqueous fractions (Pa-H2O) were 60, 70, 90 and 140 ?g/ml respectively. In CUPRAC assay percentage reduction capacity of the aqueous methanolic crude extract was highest among all other fractions. Total phenol contents of aqueous methanol extract and ethyl acetate fraction were almost comparable and indicated high phenol content. Results indicated the importance of ethyl acetate extract of P. atrosanguinea as a photoprotective agent in sunscreen preparation in the pharmaceutical industry and natural antioxidants as well. Further isolation of molecules from ethyl acetate fraction was performed using column chromatography which led to the isolation of total seven molecules out of them two were characterized namely methyl pentatetraconta-30, 32, 34, 36, 38, 40, 42-heptaenoate (VVR-I) and pentadecyl butyrate (VVR-III). VVR-I is novel compound while VVR-II is already reported in literature. Moreover, in-silico study of already reported polyphenolic compounds which are considered to be anticancer agents were also carried out using Glide docking to investigate interaction pattern with MDR receptors (MRP1 and GSTP-1) involved in cancer chemotherapy. In-silico findings suggest that rutin may be used as dual modulator for MRP-1 and GSTP1-1 mediated multidrug resistance