Browsing by Author "Asapur, Prithvi"

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    Secondary structural analysis of non-mulberry silk fibroin nanoparticles synthesized by using microwave and acetone method
    (Taylor and Francis Ltd., 2020-12-04T00:00:00) Asapur, Prithvi; Mahapatra, Santosh Kumar; Banerjee, Indrani
    The 3D structure analysis for Muga silk protein was predicted through homology modeling and Ramachandran plot with the determination of quality index for Stereo/packing and 3D quality index. Silk Nanoparticles were synthesized using conventional desolvation and microwave-assisted radiolysis method from Muga silk fibroin. The secondary structure evolution due to two different synthesis methods was investigated using FTIR and Circular Dichroism (CD) spectroscopy. The microwave synthesized nanoparticles showed enhanced ?-sheet content than desolvation synthesized nanoparticles. The conformational changes of Muga silk fibroin protein to nanoparticle followed an ordered transition from random coil to ?-helix then from ?-helix to ?-turn and from ?-turn to ?-sheets where ?-helix and ?-turn are the intermediate forms before getting stabilized to the metastable ?-sheets structure. The thermodynamics involved in secondary structure evolution was studied from CD analysis and activation energy involved in the formation of the secondary structure was determined. Communicated by Ramaswamy H. Sarma. � 2020 Informa UK Limited, trading as Taylor & Francis Group.
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    Spectroscopic analysis of Muga silk nanoparticles synthesized by microwave method
    (Blackwell Publishing Ltd, 2020-04-26T00:00:00) Asapur, Prithvi; Banerjee, Indrani; Sahare, P.D.; Mahapatra, Santosh
    Muga silk nanoparticles (MSNP) were synthesized using a microwave-assisted radiolysis method. The effect of microwave on the Muga protein secondary structures was analyzed. The evolution of the secondary structure from random coils to the ?-sheets was determined by using FTIR, circular dichroism and X-ray diffraction techniques. The results showed that Muga silk fibroin protein contained the primary structure in silk-I state. When the protein was irradiated with microwave, nanoparticle synthesis was possible having silk-II state imparting crystallinity. The silk nanoparticles were characterized by a particle size analyzer and found to be of ~240�nm in size. The optical properties of these nanoparticles were studied by UV�vis. spectroscopy and photoluminescence. For studying thermal properties, differential scanning calorimetry was performed that revealed early glass transition, which could be attributed to the presence of water and proteins. It also revealed that nanoparticles are thermally stable. Such studies are important for understanding more about the MSNP and would be beneficial for their further wide applications. � 2020 International Union of Biochemistry and Molecular Biology, Inc.