Browsing by Author "Bhargava, Arpit"

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    Design and Fabrication of a Nanobiosensor for the Detection of Cell-Free Circulating miRNAS-LncRNAS-mRNAS Triad Grid
    (American Chemical Society, 2023-10-18T00:00:00) Ratre, Pooja; Nazeer, Nazim; Bhargava, Arpit; Thareja, Suresh; Tiwari, Rajnarayan; Raghuwanshi, Vinay Singh; Mishra, Pradyumna Kumar
    The increased understanding of the competitive endogenous RNA (ceRNA) network in the onset and development of breast cancers has suggested their use as promising disease biomarkers. Keeping these RNAs as molecular targets, we designed and developed an optical nanobiosensor for specific detection of the miRNAs-LncRNAs-mRNAs triad grid in circulation. The sensor was formulated using three quantum dots (QDs), i.e., QD-705, QD-525, and GQDs. These QDs were surface-activated and modified with a target-specific probe. The results suggested the significant ability of the developed nanobiosensor to identify target RNAs in both isolated and plasma samples. Apart from the higher specificity and applicability, the assessment of the detection limit showed that the sensor could detect the target up to 1 fg concentration. After appropriate validation, the developed nanobiosensor might prove beneficial to characterizing and detecting aberrant disease-specific cell-free circulating miRNAs-lncRNAs-mRNAs. � 2023 The Authors. Published by American Chemical Society.
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    Nano-engineered vitamins as a potential epigenetic modifier against environmental air pollutants
    (Walter de Gruyter GmbH, 2022-06-20T00:00:00) Ratre, Pooja; Chauhan, Prachi; Bhargava, Arpit; Tiwari, Rajnarayan; Thareja, Suresh; Srivastava, Rupesh Kumar; Mishra, Pradyumna Kumar
    Air pollution has emerged as a serious threat to human health due to close association with spectrum of chronic ailments including cardiovascular disorders, respiratory diseases, nervous system dysfunctions, diabetes and cancer. Exposure to air-borne pollutants along with poor eating behaviours and inferior dietary quality irreversibly impacts epigenomic landscape, leading to aberrant transcriptional control of gene expression which is central to patho-physiology of non-communicable diseases. It is assumed that nutriepigenomic interventions such as vitamins can control such adverse effects through their immediate action on mitochondrial epigenomic-axis. Importantly, the exhaustive clinical utility of vitamins-interceded epigenetic synchronization is not well characterized. Therefore, improving the current limitations linked to stability and bioavailability issues in vitamin formulations is highly warranted. The present review not only sums up the available data on the role of vitamins as potential epigenetic modifiers but also discusses the importance of nano-engineered vitamins as potential epidrugs for dietary and pharmacological intervention to mitigate the long-term effects of air pollution toxicity. � 2022 Walter de Gruyter GmbH, Berlin/Boston.