Browsing by Author "Dhiman Monisha"

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Characterization of Hexaploid Wheat Protein on the Basis of their Date of Release in Indian sub- continent
(Central University of Punjab, 2018) Negi, Arti; Dhiman Monisha
Wheat is the third most grown cereal worldwide. The storage proteins of wheat represent an important source of food and energy and are also involved in the determination of bread wheat quality. These gluten proteins are categorized as prolamins, composed of monomeric gliadin (single chain polypeptides) and polymeric glutenin (multiple polypeptide chains).Unfortunately consumption of these gluten protein is known to be linked with range of clinical disorders e.g. celiac disease, wheat allergy and wheat intolerance. The main objective of the present work is to elaborate a detailed knowledge of the variability of proteins and protein fractions of the wheat varieties based on their origin. 25 wheat varieties procured from Indian Institute of Wheat and Barley Research were evaluated for analysis of total wheat protein and gluten protein by sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDS- PAGE) and cluster analysis was done based on SDS-PAGE gels to investigate variation among varieties. Multiple sequence alignment of ?-gliadin protein of different genes was done and region of highest variability among them is reported. This study shows that 25 varieties of wheat of different origin vary in their total wheat protein as well as gluten content. However the results of cluster analysis of gluten showed low degree of heterogeneity among the varieties.
Design, synthesis and biological evaluation of novel indole-xanthendione hybrids as selective estrogen receptor modulators
(Elsevier Ltd, 2018) Singla, Ramit; Gupt,a Kunj Bihari; Upadhyay, Shishir; Dhiman Monisha; Jaitak Vikas
Ground breaking clinical therapeutic advances in the treatment of breast cancer (BC) is the introduction of selective estrogen receptor modulators (SERMs). We have expeditiously designed and synthesized indole-xanthendione hybrids by coalescing the indole nucleus with xanthendione. All the compounds were first screened for anti-proliferative activity, cytotoxicity and ER-? binding affinity by utilizing ER-? dominant T47D BC cell lines, PBMCs and ER-? competitor assay kit. From this study, two representative compounds 6e and 6f showing most promising activity were advanced for gene expression studies for targeting ER-?. Cell imaging experiment undoubtedly indicate that both the compounds were able to cross cellular bio membrane and accumulate thus instigating cytotoxicity. RT-PCR and Western blotting experiments further strengthened that both compounds altered the expression of mRNA and receptor protein of ER-?, thereby forestalling downstream transactivation and signalling pathway in T47D cells line. Structural investigation from induced fit simulation study suggest that indole moiety of the compounds 6e and 6f helps in the anchoring of the xanthendione moiety in the hydrophobic region of the cavity thus enabling the compound to bind in antagonistic conformation similar to bazedoxifene by extensive hydrogen bonding and Van der Waals forces. All these finding collectively imply that compound 6e and 6f represents a novel potent ER-? antagonist and in the development of SERMs for the management of BC.