Browsing by Author "Gupta, Swati"

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    In silico phytochemical repurposing of natural molecules as entry inhibitors against RBD of the spike protein of SARS-CoV-2 using molecular docking studies
    (Inderscience Publishers, 2023-04-18T00:00:00) Gupta, Pawan; Gupta, Swati; Sinha, Sukrat; Sundaram, Shanthy; Sharma, Vishnu K.; Munshi, Anjana
    The receptor binding domain (RBD) of Spike-protein (S-protein) is responsible for virus entry via interaction with host protein ACE2 (angiotensin-converting enzyme 2), present on the cell surface of humans. Therefore, S-protein is an important target to block the entry of the SARS-CoV-2 into the cell for further growth. In the present study, phytochemical repurposing of natural molecules: narirutin, naringin, neohesperidin and hesperidin were performed against the RBD S-protein/ACE2 interface as well as the RBD of the S-protein using molecular docking. These natural molecules were found to have structural similarity to each other and had binding potential against the viral infections. It is first time reported here that the naringin and narirutin are having binding potential against both RBD S-protein/ACE2 interface and active site of RBD of S-protein using binding mode analysis. Hence, this study will open avenues for multitargeting similar natural molecules binding against the SARS-CoV-2 proteins as all reports are made in this single study. Copyright � 2023 Inderscience Enterprises Ltd.
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    Investigation for Maternal Uniparental Disomy of Chromosome 7 in a Silver Russell Syndrome patient
    (Central University of Punjab, 2018) Gupta, Swati; Khetarpal, Preeti
    Silver-Russell syndrome (SRS) is a genetically heterogeneous disorder. Individuals with SRS show clinical features with varying severity. The major criteria for clinical diagnosis of SRS are intrauterine growth retardation (IUGR) accompanied with post natal growth retardation (PNGR) and relative macrocephaly, triangular face, feeding difficulties, fifth finger clinodactyly. Maternal Uniparental disomy of chromosome 7 had been implicated in 10% of SRS cases; in about 1% cases, structural chromosomal aberrations has been reported and in about 45% cases, epimutation have been detected in Imprinting control region (ICR1) of 11p region. Aetiology of remaining cases unknown. To investigate Maternal Uniparental Disomy of chromosome 7 in a Silver Russell Syndrome patient. The sample was collected of female patient suggested of SRS, clinically diagnosed with relative macrocephaly, mild facial asymmetry with postnatal growth retardation. The present study had been undertaken with an objective to detect maternal Uniparental disomy of chromosome 7 using locus specific primers to amplify STR loci by PCR. PCR products were visualized on 7.5% native Polyacrylamide Gel Electrophoresis (PAGE). On analysing the gel, matUPD7 was ruled out. Patient recruited in this study was an isolated cases with no previous incidence in the family.