Browsing by Author "Kaur, Inderjeet"

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    Nanoemulsions (O/W) prepared from essential oil extracted from Melaleuca alternifolia: synthesis, characterization, stability and evaluation of anticancerous, anti-oxidant, anti-inflammatory and antidiabetic activities
    (Taylor and Francis Ltd., 2023-08-28T00:00:00) Sharma, Arun Dev; Chhabra, Ravindresh; Jain, Puneet; Kaur, Inderjeet; Amrita; Bhawna
    Essential oil from Melaleuca alternifolia (also known as Tea tree essential oil, TTO) is used as traditional medicine and used as therapeutic in medicine, food and cosmetic sectors. However, this oil is highly unstable, volatile and prone to oxidation which limits its practical use. The objective of this study was synthesis of tea tree oil based O/W (oil/water) nanoemulsions (tea tree essential oil nanoemulsions, TNE) and evaluation of its biological potential. Physiological characterization was carried out using UV, fluorescent, and FT-IR techniques. Various biological activities such as anticancerous, antidiabetic and anti-inflammatory were also estimated. Pharmacokinetics study on TNE was carried out. Encapsulation efficiency of nanoemulsions was found to be 83%. Nanoemulsions were spherical in shape with globule size 308 nm, zeta potential ?9.42 and polydispersity index was 0.31. Nanoemulsions were stable even after 50 days of storage at different temperatures. Anti-oxidant potential of TNE was conducted by various assays and IC50 were: Nitric oxide radical scavenging activity:225.1, DPPH radical scavenging activity:30.66, Iron chelating assay:38.73, and Iron reducing assay:39.36. Notable anticancer activity was observed with the percent cell viability of HeLa cells after treatment with 1, 2 and 5 �l of TNE was 82%, 41% and 24%, respectively. Antidiabetic study revealed that TNE inhibited -amylase in a dose-dependent manner, with 88% inhibition at its higher volume of 250 �l. Drug kinetic study revealed that nanoemulsions exhibited first-order model. Based on this, the possible role of M. alternifolia oil-based nanoemulsions in cosmetic, food, and pharma sectors has been discussed. � 2023 Informa UK Limited, trading as Taylor & Francis Group.
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    Preparation, Characterization, and Biological Potential of Nanoemulsion from Rosmarinus officinalis L. Essential Oil
    (Springer, 2023-10-02T00:00:00) Sharma, Arun Dev; Chhabra, Ravindresh; Jain, Puneet; Kaur, Inderjeet; Chauhan, Amrita; Rani, Retika
    Essential oil from Rosmarinus officinalis (also known as rosemary essential oil) is used as traditional medicine and used as therapeutic in medicine, food, and cosmetic sectors. However, this oil is highly unstable, volatile, and prone to oxidation which limits its use. Thus, encapsulation is the better way to protect this oil from adverse conditions. The objective of this study was synthesis of rosemary essential oil (REO) based O/W (oil/water) nanoemulsions (designated as RNE) and evaluation of its biological potential. Physiological characterization was carried out using, UV, fluorescent, and FT-IR techniques. Various biological activities such as anticancerous, antidiabetic, and anti-inflammatory were also estimated. Pharmacokinetics studies on RNE were carried out. Encapsulation efficiency of RNE was found to be 92%. RNE nanoemulsions were spherical in shape with globule size, Z-Average (nm) size 220 nm, zeta potential ?11.33 mV, and polydispersity index was 0.47. RNE nanoemulsions were stable even after 50 days of storage at different temperatures. Antioxidant potential of RNE was conducted by various assays and IC50 were DPPH free radical scavenging activity: 21.53, nitric oxide radical scavenging activity: 26.66, hydroxyl radical scavenging activity: 32.69, ABTS radical scavenging activity: 33.05, and iron chelating assay: 38.78. Notable anticancer activity was observed with the percent cell viability of HeLa cells after treatment with RNE was 23% at higher concentration of 5 ?g. Antidiabetic study revealed that RNE inhibited ?-amylase in a concentration dependent manner, with 71% inhibition at its higher concentration of 250 ?g. RNE depicted maximum antibacterial activity against Bacillus subtilis at higher concentration of 300 ?g. Drug kinetic study revealed that nanoemulsions exhibited Korsmeyer�Peppas model. Based on this, the possible role of R. officinalis oil-based nanoemulsions in food, cosmetic, and pharma sectors has been discussed. � 2023, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.