Browsing by Author "Kaur G."

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Evaluation of the antioxidant, antibacterial and anticancer (lung cancer cell line A549) activity of: Punica granatum mediated silver nanoparticles
(Royal Society of Chemistry, 2018) Annu; Ahmed S.; Kaur G.; Sharma P.; Singh S.; Ikram S.
This work aimed to synthesize silver nanoparticles via an environmentally benign route, using the aqueous extract of Punica granatum as a precursor as well as a stabilizing and reducing agent. The as-synthesized silver nanoparticles were confirmed using UV-visible spectroscopy with an absorbance peak at 450 nm and were thereafter further confirmed using dynamic light scattering (DLS), High Resolution Transmission Electron Microscopy (HR-TEM) and X-Ray Diffraction (XRD). TEM analysis revealed 6-45 nm and spherically dispersed nanoparticles and XRD showed the crystalline nature of the nanoparticles. The free radical scavenging activity of the nanoparticles for DPPH and intracellular reactive oxidative species (ROS) production were observed using dihydroethidium (DHE) non-fluorescent stain and a CellROX® Deep Red fluorescent probe. Antibacterial assays against the most common Gram negative (Escherichia coli) and Gram positive (Staphylococcus aureus) bacteria showed a higher zone of inhibition against S. aureus. Furthermore, the anti-cancerous activity of the biologically synthesized silver nanoparticles was revealed by the inhibited cell growth of lung cancer A549 cells and no cytotoxicity was observed. This may be due to their ability to arrest the cell cycle at G1 phase. Thus, this work provides a gateway to explore more about the anticancer properties of biogenically synthesized silver nanoparticles and these biologically prepared silver nanoparticles have the potential to be utilized in biomedical science.
Fake news detection of Indian and United States election data using machine learning algorithm
(Blue Eyes Intelligence Engineering and Sciences Publication, 2019) Kumar A.; Singh S.; Kaur G.
The world of digital media is thriving by the day and hence, there is an urge of businesses to magnify it more gaining them maximum financial benefits. This particular urge calls for more and more expansions concerning creating and developing new content whether it's in the form of websites that aims at branding businesses or could be in the form of online newspapers and magazines. Since from last few decades’ medium of communication had changed. Now a day people are using social networks very extensively for news updates. These networks aim to make social lives better. Today, everyone knows and uses social media which contains unverified article, post, message and news. Nowadays' fake news is making various issues from mocking articles to a created news and plan government publicity in certain outlets. Fake news and the absence of trust in the media are developing issues with immense consequences in our general public. It is needed to look into how the techniques in the fields of computer science using machine learning, natural language processing helps us to detect fake news. Fake news is now observed as one of the major threats to freedom of expression, journalism, and democracy of a country. In this research, a comprehensive way of detecting fake news using machine learning model has been presented that is trained by Fake News data based on US election and trained on recent Indian political Fake news.
Ground/drinking water contaminants and cancer incidence: A case study of rural areas of South West Punjab, India
(Taylor and Francis Inc., 2019) Kaur G.; Kumar R.; Mittal S.; Sahoo P.K.; Vaid U.
This study was carried out in the rural areas of South West Punjab, India, to evaluate the groundwater quality and cancer incidence. The epidemiological study was carried using standardized questionnaire method, and the groundwater samples were analyzed for heavy metals by ICP-MS and AAS. The results showed that the cancer prevalence was highest in the age group of > 60, followed by >45–60 years old in both males and females. The average cancer rate in females (272 cases/lakh) was ?3 times higher than the India's national cancer average of 80 cases/lakh. The mean concentration of As(27.59 µg/L), Pb (48.3 µg/L), U (96.56 µg/L), NO3– (67.32 mg/L), and F– (4.7 mg/L) exceeded the drinking water limits of WHO/BIS. Health risk analysis indicated that As, Pb, U, and F– with NO3– are the major groundwater contaminants, which may be one of the potential cause of cancer incidences. Multivariate analyses reveal that anthropogenic activities are source of NO3–, whereas U, As, and F– are mainly of geogenic origin. The carcinogenic and non-carcinogenic risk followed in the order of As > Pb and U > F–>NO3–>Cu > Zn, respectively. Further, correlations between cancer incidence and groundwater quality have been discussed.
Health risk assessment of occupationally pesticide-exposed population of cancer prone area of punjab
(Oxford University Press, 2018) Kaur G.; Dogra N.; Singh S.
The alarming health issues especially the unusually high number of cancer cases in agriculture community of Bathinda district of Punjab (India) is a serious concern. There is limited knowledge about the role of gene-environment interactions in oncogenesis prevalent in this area. The aim of this study was to evaluate the association of oxidative stress with CYP1A2, CYP2B6, CYP2C9, CYP3A4, and PON1 genetic variation in the pesticide-exposed (occupationally) population of Bathinda district of Punjab (India). This study demonstrated significantly elevated relative risk (RR) of lower antioxidant defense mechanism (Glutathione, Catalase, Superoxide Dismutase, Glutathione peroxidases, and Glutathione Reductase) in occupationally pesticide-exposed group (n = 120) as compared with unexposed group (n = 84) from Bathinda district of Punjab (India). Our data shows pesticide exposure to be a major risk factor leading to increased oxidative stress inside the body. Gas chromatographic analysis revealed the residues of organophosphates (chlorpyriphos, dichlorvos, ethoprophos) and herbicides (atrazine, butachlor, alachlor, metolachlor) in the blood samples of the exposed population. In vitro results showed a dose dependent decrease in cell viability following treatment of pesticides detected in blood samples in hPBMCs and A549 cell line. Genetic variation analysis revealed missense mutations in CYP2B6 (2 mutations), CY3A4 (1 mutation), and CYP2C9 (2 mutations). The observed mutations have been predicted to cause structural and conformation change in protein structure which could result in altered stability. In first of its kind of study, our data reveal oxidative stress and pesticide residue accumulation inside the body to be the major reasons for health concerns in Bathinda district.
P53-mediated anticancer activity of Citrullus colocynthis extracts
(Bentham Science Publishers, 2019) Joshi G.; Kaur J.; Sharma P.; Kaur G.; Bhandari Y.; Kumar R.; Singh S.
Background: Current anticancer therapeutics comes with significant side effects and thus focus is shifting towards minimizing the side effects or to avoid the disease altogether. Thus, various natural products are being investigated for their potential therapeutic values which can be easily included in daily diet of a person. Citrullus colocynthis (L.) fruit is commonly used in traditional medicines and is known to have antioxidant effects, thus may possess potent anticancer activity as well. Objectives: To establish the anticancer potential of fruit belonging to Citrullus colocynthis (L.) and delineate the potential targets. Results: In the present study it was found that seed and pulp extracts of the fruit are effective against various cancer cell lines while the normal cells, with lower rate of division, remain largely unaffected. The current study for the first time shows that these extracts function via regulation of p53 pathways and the mode of apoptosis is mostly via mitochondrial (intrinsic) pathway. The biological profiling of the extracts was also validated using molecular modelling studies utilizing the two major polyphenols constituents from colocynths i.e., Isoorientin and Isovitexin. Conclusion: The study suggested that the constituent has a multiple target approach for the inhibition of cancer cell proliferation and inhibition of ROS production via the major apoptotic proteins. All of these outcomes suggest and establish a critical role of ROS accumulation and mitochondrial function in the p53-dependent cell.