Browsing by Author "Kaur J."

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Control of sludge microbial biofilm by novel quorum quenching bacteria Pseudomonas nitroreducens JYQ3 and Pseudomonas JYQ4 encapsulated sodium alginate - Magnetic iron nanocomposites
(Elsevier Ltd, 2018) Kaur J.; Yogalakshmi K.N.
Biofouling control via quorum quenching (QQ) approach is becoming popular among other fouling mitigation strategies. In the present study, the mitigation of membrane biofouling problem via disruption of cell-to-cell communication was studied by immobilizing QQ bacteria in sodium alginate magnetic nanocomposite beads. Two QQ bacteria viz. Pseudomonas nitroreducens JYQ3 and Pseudomonas JYQ4 isolated from dairy waste activated sludge was immobilized individually into the beads. Scanning electron microscopy (SEM) analysis revealed the successful immobilization of QQ bacteria into the alginate nanoparticle beads. The Confocal laser scanning microscopy (CLSM) results provided evidence of biofouling controlled activities of Pseudomonas nitroreducens JYQ3 and Pseudomonas JYQ4 immobilized nanocomposite (IMN) beads. Further, the membranes incubated with Pseudomonas nitroreducens JYQ3 and Pseudomonas JYQ4 IMN beads showed better flux (19% and 22% higher, respectively) than the control. Hence, the results revealed that the QQ bacteria IMN beads would be an efficient approach for controlling the biofilm developed on the cellulose acetate membrane surface.
P53-mediated anticancer activity of Citrullus colocynthis extracts
(Bentham Science Publishers, 2019) Joshi G.; Kaur J.; Sharma P.; Kaur G.; Bhandari Y.; Kumar R.; Singh S.
Background: Current anticancer therapeutics comes with significant side effects and thus focus is shifting towards minimizing the side effects or to avoid the disease altogether. Thus, various natural products are being investigated for their potential therapeutic values which can be easily included in daily diet of a person. Citrullus colocynthis (L.) fruit is commonly used in traditional medicines and is known to have antioxidant effects, thus may possess potent anticancer activity as well. Objectives: To establish the anticancer potential of fruit belonging to Citrullus colocynthis (L.) and delineate the potential targets. Results: In the present study it was found that seed and pulp extracts of the fruit are effective against various cancer cell lines while the normal cells, with lower rate of division, remain largely unaffected. The current study for the first time shows that these extracts function via regulation of p53 pathways and the mode of apoptosis is mostly via mitochondrial (intrinsic) pathway. The biological profiling of the extracts was also validated using molecular modelling studies utilizing the two major polyphenols constituents from colocynths i.e., Isoorientin and Isovitexin. Conclusion: The study suggested that the constituent has a multiple target approach for the inhibition of cancer cell proliferation and inhibition of ROS production via the major apoptotic proteins. All of these outcomes suggest and establish a critical role of ROS accumulation and mitochondrial function in the p53-dependent cell.
Screening of quorum quenching activity of the bacteria isolated from dairy industry waste activated sludge
(Center for Environmental and Energy Research and Studies, 2019) Kaur J.; Yogalakshmi K.N.
Intercellular bacterial communication process via exchange of signalling molecules acyl homoserine lactone results in various group activities like bioluminescence, antibiotic production, biofilm formation, sporulation, and virulence. The signalling molecules are targeted, and the communication is interrupted by a group of bacteria termed quorum quenching bacteria. The present study aims to isolate the quorum quenching bacteria from the waste activated sludge collected from the dairy industry effluent treatment plant and explore for its quorum quenching potential. The bacteria were cultured in the KG medium containing n-hexanoyl homoserine lactone as a sole source of carbon and nitrogen. The isolates were identified by the 16S ribosomal deoxyribonucleic acid analysis and subsequently were evaluated for its quorum quenching activity through Chromobacterium violaceum CV026 biosensor assay. The n-hexanoyl homoserine lactone degradation was quantified by GC–MS analysis. The 16S ribosomal deoxyribonucleic acid analysis revealed the isolated bacteria as Klebsiella pneumoniae (JYQ1 and JYQ5), Acinetobacter baumannii JYQ2, Pseudomonas nitroreducens JYQ3, and Pseudomonas JYQ4. The biosensor strain assay and GC–MS analysis indicated that all the isolates possessed an inherent ability to degrade N-hexanoyl homoserine lactone. The strain Pseudomonas JYQ4 exhibited the highest quorum quenching activity of 84 ± 3.3% within 6 h of incubation. The strain A. baumannii JYQ2 acted both as quorum sensing and as quorum quenching bacteria as evidenced by the decrease in quorum quenching from 79 ± 3.1 to 76.8 ± 2.5%.