Browsing by Author "Kumar, Rajeev"
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Item Phytogenetic analysis of bacterial diversity of heavy metal affected(Central University of Punjab, 2012) Kumar, Rajeev; Kumar, SanjeevThe soil of Bathinda region is affected by heavy metals due to anthropogenic activity, industrial effluent and fly ash. Three soil samples in triplicate were collected from three different places of Bathinda region, abandoned soil, agricultural soil, thermal power plant fly ash soil. Based on 16S rRNA gene cloning and sequence analysis, phylogentic analysis of bacterial diversity was performed. 366 clones were picked and through colony PCR 128 positive clones were chosen. Out of 128 clones, 126 clones were sequenced in three fragments and all three fragments aligned using SeqMan Lasergene ver.10.0 (DNA STAR) software. BLAST analysis of sequenced and aligned samples was carried out at NCBI to find out the homology with different groups of bacteria. All 126 clones aligned for phylogenetic analysis by MEGA 5 (Neighbor Joining method). 32 genus belong to 8 phyla were observed in all three samples. Most dominant phylum is Proteobacteria followed by Bacteriodates. Pseudomonas spp. is dominant in agricultural and thermal power plant soil. Abandoned soil showed maximum diversity followed by thermal power plant soil. In agricultural soil minimum diversity was observed. So with this investigation it can be concluded that lands without anthropogenic activities are rich in bacterial diversity as compared to more anthropogenic active areas.Item Video quality evaluation using DWT-SPIHT based watermarking technique(Institute of Electrical and Electronics Engineers Inc., 2018) Kumar, Rajeev; Kumar, Sanjeet; Brar, Sukhreet SinghThe video quality analysis is very important in today's video broadcasting and transmission quality control because the quality is a key factor in customer satisfaction. This paper presents a full reference based video quality evaluation technique, using the application of watermarking. Frames with low complexity value are selected for the insertion of the watermark, on the selected video frames; DWT is applied up to third level to get the DWT coefficients. Then SPIHT encoding/decoding is applied which helps in finding the significant coefficients for the insertion of watermark. The significant coefficients are converted into bit-planes and 3rd bit plane (from LSB side) of each coefficient is selected for watermark. The SPIHT algorithm ensures that the watermark bits are inserted uniformly throughout the frames. After embedding the watermark, the IDWT is applied and we get the watermarked frames which are then incorporated in the video file. The simulation results show no visible difference between watermarked frame and original frame. The video quality is estimated in terms of peak signal to noise ratio (PSNR), structure similarity (SSIM) and true detection rates (TDR) against various attacks like Salt pepper noise, rotation Gaussian noise and compression attacks. The results indicate that the proposed technique for quality evaluation of the video, accurately calculates the quality of a video and also it is robust against various attacks. ? 2016 IEEE.