Browsing by Author "Kumar P."

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    Evaluation of heavy metals toxicity in the groundwater of some villages of Sirsa district of Haryana, India
    (Rasayan Journal of Chemistry, c/o Dr. Pratima Sharma, 2019) Kumar P.; Jain S.; Kumar B.
    An attempt was made to assess the groundwater quality in some rural parts of Sirsa district of Haryana, India with respect to heavy metals contamination. For this purpose, 23 groundwater samples were collected from the bore wells during pre-monsoon in 2017 from some selected locations in the study area where the groundwater is used for drinking and agricultural activities. The samples have been analyzed for seven heavy metals viz. Mn, As, Zn, Cd, Pb, Cu and Cr using ICP-MS. The results were compared with the BIS standards to assess the suitability of groundwater for drinking. To assess the heavy metal contamination, the heavy metal pollution index (HPI), metal index (MI) and hazard index (HI) was calculated. The groundwater with HI > 1 falls in the high pollution category. In our study, 10 out of 23 samples were not fit to consume by the infants and all the 23 samples were found to be unfit for both the children and the adults. The correlation matrix showed a good correlation of HPI with Mn (r = 0.998), As (r = 0.993), Cu (r = 0.998), Cr (r = 0.998) and with Pb (r = 0.998) whereas the correlation of MI with Mn (r = 0.998), As (r = 0.994), Cu (r = 0.998), Cr (r = 0.999) and with Pb (r = 0.998).
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    Pivotal role of bZIPs in amylose biosynthesis by genome survey and transcriptome analysis in wheat (Triticum aestivum L.) mutants
    (Nature Publishing Group, 2018) Kumar P.; Mishra A.; Sharma H.; Sharma D.; Rahim M.S.; Sharma M.; Parveen A.; Jain P.; Verma S.K.; Rishi V.; Roy J.
    Starch makes up 70% of the wheat grain, and is an important source of calories for humans, however, the overconsumption of wheat starch may contribute to nutrition-associated health problems. The challenge is to develop resistant starch including high amylose wheat varieties with health benefits. Adapting advance genomic approaches in EMS-induced mutant lines differing in amylose content, basic leucine zipper (bZIP) regulatory factors that may play role in controlling amylose biosynthesis were identified in wheat. bZIP transcription factors are key regulators of starch biosynthesis genes in rice and maize, but their role in regulating these genes in wheat is poorly understood. A genome-wide survey identified 370 wheat bZIPs, clustered in 11 groups, showing variations in amino acids composition and predicted physicochemical properties. Three approaches namely, whole transcriptome sequencing, qRT-PCR, and correlation analysis in contrasting high and low amylose mutants and their parent line identified 24 candidate bZIP (positive and negative regulators), suggesting bZIPs role in high amylose biosynthesis. bZIPs positive role in high amylose biosynthesis is not known. In silico interactome studies of candidate wheat bZIP homologs in Arabidopsis and rice identified their putative functional role. The identified bZIPs are involved in stress-related pathways, flower and seed development, and starch biosynthesis. An in-depth analysis of molecular mechanism of novel candidate bZIPs may help in raising and improving high amylose wheat varieties. - 2018, The Author(s).