Browsing by Author "Mahapatra S.K."

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    Effect of TiO2 and Fe doped TiO2 nanoparticles on mitochondrial membrane potential in HBL-100 cells
    (American Institute of Physics Inc., 2019) Barkhade T.; Mishra S.; Chander H.; Mahapatra S.K.; Banerjee I.
    Titanium dioxide (TiO2) nanoparticles (NPs) have made unbelievable progress in the field of nanotechnology and biomedical research. The proper toxicological assessment of TiO2 NPs and the reduction of its cytotoxicity need to be addressed. Fe doping in TiO2 has been investigated to reduce the toxic effects of TiO2 NPs. Fe doped TiO2 powder samples were synthesized by sol-gel methods. The prepared samples were characterized by x-ray diffractometer (XRD), transmission electron microscope (TEM), and Raman spectroscopy to study their structure, morphology, and molecular conformation. XRD results revealed the coexistence of anatase (A) and rutile (R) phases of TiO2. The A-R transformation was observed with an increase in Fe doping along with the formation of ?-Fe2O3 phase. TEM showed changes in morphology from spherical nanoparticles to elongated rod-shaped nanostructures with increasing Fe content. Shape variation of TiO2 nanoparticles after incorporation of Fe is a key reason behind the toxicity reduction. The authors observed that the toxicity of TiO2 nanoparticles was rescued upon Fe incorporation. The effect of NPs on the mitochondrial membrane potential (MMP) was assessed using flow cytometry. The MMP (%) decreased in TiO2 treated cells and increased by 1% Fe doped TiO2 NPs treated cells. Confocal imaging revealed the presence of functional mitochondria upon the exposure of Fe doped TiO2 NPs. The goal of the present study was to decrease the toxic effects induced by TiO2 NPs on mitochondrial potential and its prevention by Fe doping.
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    Electron beam induced synthesis of Ru-rGO and its super capacitive behavior
    (IOP Publishing Ltd, 2019) Saykar N.G.; Phatangare A.; Banerjee I.; Bhoraskar V.N.; Ray A.K.; Mahapatra S.K.
    We report an in situ synthesis of ruthenium-reduced graphene oxide (Ru-rGO) using 6 MeV electron beam assisted radiolytic reduction method and its supercapacitive behavior. X-ray diffraction (XRD), transmission electron microscopy (TEM) and field emission scanning electron microscopy (FESEM) explore Ru nanoparticles of size ?2 nm are decorated on rGO sheets. Raman spectroscopy shows I D/I G ratio increased and formation of bilayer rGO after electron beam irradiation. The defect density in Ru-rGO is increased due to the electron beam irradiation as compared to its counterpart GO. The Ru-rGO based supercapacitor exhibits specific capacitance (128.1 ± 5.59) F g-1 at 10 mV s-1 scan rate. The specific capacitance retention of Ru-rGO is up to 99.4% at 900 cycles while it increases to 130% at 5000 cycles. Discharge curve of the supercapacitor involves three current decay processes viz. activation polarization, ohmic polarization and concentration polarization. The highest energy density of (4.125 ± 0.19) W h kg-1 and power density of 1.44 kW kg-1 are achieved with Ru-rGO supercapacitor. This unique electron beam assisted techniques illustrates a promising method of the fabrication of high performance supercapacitor.
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    Nano-bio interface study between Fe content TiO2 nanoparticles and adenosine triphosphate biomolecules
    (John Wiley and Sons Ltd, 2019) Barkhade T.; Phatangare A.; Dahiwale S.; Mahapatra S.K.; Banerjee I.
    The advent of nano-biotechnology has inspired the interface interaction study between engineered nanoparticles (NPs) and biomolecules. The interaction between Fe content titanium dioxide (TiO2) NPs and adenosine triphosphate (ATP) biomolecules has been envisioned. The effect of Fe content in TiO2 matrix was studied using X-ray diffraction (XRD) and transmission electron microscopy (TEM). The increase in Fe content caused a decrease in particle size with change in morphology from spherical to one-dimensional rod structure. The Fe incorporation in the TiO2 matrix reduced the transition temperature from anatase to rutile (A-R) phase along with formation of haematite phase of iron oxide at 400°C. The interaction of Fe content TiO2 NPs with ATP molecule has been studied using spectroscopic method of Raman scattering and infrared vibration spectrum along with TEM. Fe content in TiO2 has enhanced the interaction efficiency of the NPs with ATP biomolecules. Raman spectroscopy confirms that the NPs interact strongly with nitrogen (N7) site in the adenine ring of ATP biomolecule. Engineering of Fe content TiO2 NP could successfully tune the coordination between metal oxide NPs with biomolecules, which could help in designing devices for biomedical applications.
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    Study of mitochondrial swelling, membrane fluidity and ROS production induced by nano-TiO2 and prevented by Fe incorporation
    (Royal Society of Chemistry, 2019) Barkhade T.; Mahapatra S.K.; Banerjee I.
    The potential impact of TiO2 and Fe incorporated TiO2 nanoparticles at the organelle level has been reported. The toxicity of the samples on mitochondria isolated from chicken liver tissue has been examined through mitochondrial swelling, membrane fluidity, ROS generation capacity, and activity of complex II. The toxic effect of TiO2 was prevented by incorporating Fe into the TiO2 matrix at different concentrations. The activity of the succinate dehydrogenase enzyme complex was affected and permeabilization of the mitochondrial inner membrane to H+ and K+ and its alteration in membrane fluidity at 100 ?g mL-1 of nano-TiO2 dosage were investigated, which showed significant changes in the anisotropy of DPH-labeled mitochondria. Fe incorporation into the TiO2 matrix makes it more biocompatible by changing its structure and morphology.