Browsing by Author "Sahoo, Suban K."

Filter results by typing the first few letters
Now showing 1 - 2 of 2
  • No Thumbnail Available
    Item
    Synthesis of thiolated chlorogenic acid-capped silver nanoparticles for the effective dual action towards antimicrobial and anticancer therapy
    (Springer Science and Business Media Deutschland GmbH, 2022-07-22T00:00:00) Nayak, Jyotsnamayee; Prajapati, Kumari Sunita; Kumar, Shashank; Sahoo, Suban K.; Kumar, Rajender
    Chlorogenic acid (CGA) was thiolated using Traut�s reagent. Then, thiolated chlorogenic acid (CAT) functionalized on the silver nanoparticles (AgNPs). The resulting CAT-functionalized silver nanoparticles (CAT-AgNPs) have higher stability with a uniform spherical shape compared to naked AgNPs. The average particle size is 10�nm, as revealed by transmission electron microscopy (TEM). The antimicrobial activity was higher against both gram-positive and gram-negative bacteria of spherically stable nanoparticles evaluated by the disk diffusion method. Minimal inhibitory concentration (MIC) values for CAT-AgNPs were 130��g/ml for Staphylococcus aureus and 150��g/ml for Escherichia coli. Cytotoxicity studies showed that the CAT-AgNPs show maximum activity against MCF-07 cell lines with an IC50 value of 20�ng/ml. Compared to traditional noncovalent interactions, the Ag�S covalent linkage of CAT with AgNPs helped in highly stable capping. The prepared CAT-AgNPs show anticancer and antibacterial activities, thus providing an efficient antimicrobial nano-drug carrier. Nanoparticles with a dual-mode of action help in preventing fouling of nanocarriers while delivering drugs to targeted cells, opening up many applications in nanobiotechnology and nanomedicine. � 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
  • No Thumbnail Available
    Item
    Thiolated ?-cyclodextrin modified iron oxide nanoparticles for effective targeted cancer therapy
    (Elsevier Ltd, 2022-10-13T00:00:00) Nayak, Jyotsnamayee; Prajapati, Kumari Sunita; Kumar, Shashank; Vashistha, Vinod Kumar; Sahoo, Suban K.; Kumar, Rajender
    Inspired by the mitochondria iron-sulfur protein, direct functionalization of thiolated ?-cyclodextrin (?-CD-SH) on iron oxide nanoparticles (IONPs) through Fe-S bond was done. The resulting system had an average size of 14 nm with an ellipsoidal shape. The X-Ray photoelectron spectroscopy (XPS) confirmed the formation of the Fe-S bond. Doxorubicin (DOX) was chosen as a model drug, about 12.45 �M/mg entrapped in ?-CD-SH coated IONPs (T?CD-IONPs). The hybrid nanocarrier possessed high stability and drug loading efficiency. The invitro release data revealed an overall sustainable release profile without initial bust. The Higuchi kinetic model best fits the release mechanism, based on diffusion action in dosages proportional to the square root of time. The surface coating and particle size have a crucial role in the cellular responses and effective toxic mechanisms. The cellular internalization of drug-loaded nanoparticles (NPs) into the breast cancer cell line MCF-07 was done using MTT assay and confocal imaging. The prepared system shows high performance with an IC50 value at 67 nM of nanoparticle concentration. The prepared nanoparticles are promising candidates for the effective targeted delivery of hydrophobic drugs with enhanced theragnostic activity. � 2022 Elsevier Ltd