Browsing by Author "Wusirika, Ramakrishna"

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    Bioinformatic Analysis Of Four Proteins Differentially Expressed In A Colon Cancer Cell Line Treated With Rice Callus Suspension Culture
    (Central University of Punjab, 2018) Banerjee, Aditi; Wusirika, Ramakrishna
    Plant callus extracts have been shown to have effective anticancer activity. In a previous study, cancer cells (colon cancer cell line SW620), was treated with rice callus suspension culture and certain proteins were found to be differentially regulated (9 upregulated proteins and 3 downregulated proteins). Bioinformatic analysis was done for three upregulated and one downregulated proteins to elucidate the molecular mechanism of cancer progression in the RCSC treated cells. NCBI, STRING, KEGG, PDB and miRNA database searches were performed for the four proteins. Information about the gene size, chromosomal location, protein structure, protein interactions, and pathways was extracted and analyzed. Based on the previously mentioned attributes, possible role of upregulated proteins (Liprin?1, OFD1, SPINK5) and downregulated protein (Vinculin) in progression of cancer was predicted.
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    Nutrient Analysis of Two Chickpea Cultivars Grown in Local Soil and Treated with Plant Growth Promoting Bacteria from Bathinda Region
    (Central University of Punjab, 2018) Dogra, Nitin; Wusirika, Ramakrishna
    The bacteria found near plant roots are known to affect plant and soil health. Several important characteristics, such as biological nitrogen fixation, phosphate solubilization and production of siderophores and phytohormones are together called plant growth promoting (PGP) traits. In the current study, the plant growth promoting activity of five bacterial strains, Pseudomonas citronellis (PC), Pseudomonas sp. RA6, S2, Serratia marcescens CDP-13 and Symbion-K (Frateuria aurantia) were observed on chickpea varieties PBG1 and PBG5, grown in pots containing soil collected from Central University of Punjab city campus. The plants were harvested after 30 days and shoot length, root length, plant biomass and nutrient content of shoots were evaluated.
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    Pseudomonas citronellolis; a multi-metal resistant and potential plant growth promoter against arsenic (V) stress in chickpea
    (Elsevier, 2019) Adhikary, Arindam; Kumar, R; Pandir,R; Bhardwaj, Pankaj; Wusirika, Ramakrishna; Kumar, Sanjeev
    Soil micro-biota plays a vital role in maintaining plant growth and fitness under normal and adverse conditions. Pseudomonas is one of the most important free-living and copious genera in south-west Punjab and involved in plant growth promotion under heavy metal stress. In this study, we have studied microbial diversity of the agricultural and marginal land based on 16S rRNA gene and screened eight strains of Pseudomonas for its tolerances towards various heavy metals and for plant growth promoting properties (PGP). The best strain is tested in chickpea plants against Arsenic (As5+) stress. All the strains responded differently to heavy metals viz. Arsenic, (As5+ (0.3–0.5M) and As3+ (250 μg mL−1) Cadmium (Cd2+) (250–350 μg mL−1), Chromium (Cr2+) (200–350 μg mL−1) and Mercury (Hg2+) (1–2 μg mL−1). Out of eight strains, only two strains (KM594398 and KM594397) showed plant growth promoting characters, concurrently they were highly tolerant to Arsenic (As5+). Pseudomonas citronellolis (PC) (KM594397) showed the best results in terms of As5+ tolerance and plant growth promoting activity, hence further tested for actual plant growth response in chickpea (Cicer arietinum L.) under As5+ (10–160 mg kg−1) stress. Pseudomonas citronellolis enhanced plant growth and dry biomass under As5+ stress. High As5+ tolerance and plant growth promoting activity of Pseudomonas citronellolis in chickpea especially designate this strain suitable for marginal lands and heavy metals contaminated sites. © 2019 Elsevier Masson SAS
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    Role of Plant Growth Promoting Bacteria in Improving the Quality of Marginal Soil for Multiple Purposes
    (Central University of Punjab, 2018) Kumari, Ritu; Wusirika, Ramakrishna
    Degrading quality of soil by the presence of xenobiotics or change in the natural environment of soil is of great concern. One of them is heavy metal pollution of soil. With increasing population, sustainable food production is a challenge in this climate changing and water deteriorating environment. Marginal soil has been defined from different perspectives. In general, marginal land can be defined as arid and inhospitable land that has low inherent productivity. Marginal land is a good source for the production of biofuels. This can be achieved with the help of Plant Growth Promoting Bacteria (PGPB). This project provides a review of marginal soil and how PGPB can be used to improve its quality, in phytoremediation, and in the production of bioenergy and food.