Environmental Science And Technology - Master Dissertation

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Browsing Environmental Science And Technology - Master Dissertation by Subject "eugenol and ?-caryophylle: ?-cyclodextrin"

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    Encapsulation of Monoterpene and Sesquiterpene to Enhance their Biological Properties along with Physico-chemical Characterization
    (Central University of Punjab, 2018) Radha, Anu; Mittal, Sunil
    Essential oils are secondary metabolites that are basically involved in defense mechanism of plants. They have several properties in different sectors like perfume industry, pharmaceuticals etc. Moreover, their biological properties have been reported against bacteria, virus, fungi, nematodes and other pests and pathogens of crops. However, their beneficial properties cannot be explored due to their volatile nature, thermal instabilityand rapid oxidation on exposure to air. Therefore, the present work was carried out to encapsulate the essential oil constituents i.e., eugenol and ?-caryophyllene. Encapsulation was carried out using biodegradable polymer i.e. ?-cyclodextrin. The encapsulated material was further subjected to physico-chemical characterization to verify the encapsulation of guest material inside the host material. Peaks obtained in GC-MS analysis using DCM as solvent confirmed the presence of eugenol and ?-caryophyllene. ?maxfor eugenol and?-caryophyllene was observed at 280 nm and 230 nm using UV-Vis spectrophotometer.Encapsulation of eugenol and ?-caryophyllene was also confirmed by taking absorbance at 280 nm and 230 nm, respectively. On the other hand, ?-cyclodextrin (control) didn't show absorbance at 200-400 nm. Surface Electron Microscopy (SEM) revealed the morphology of the encapsulated material with rough surface, cracks and sharp edges. Encapsulated particles were reported to have size in micrometers via SEM and were therefore, referred as microparticles. The most informative technique i.e., Fourier Transform Infra-Red Spectroscopy, alsoconfirmed the interaction of guest and host molecule. The FTIR spectrum of eugenol showed characteristic peaks at 3516 cm-1 (OH), 2842-3000 cm-1 (C-H stretching), and 1511 cm-1, 1611 cm-1 and 1638 cm-1 (C=C aromatic ring). The FTIR spectrum of ?-caryophyllene showed bands at 3067-2856 cm-1 and 1671-885 cm-1. Thermal property of eugenol and ?-caryophyllene was also improved by encapsulation. Thermogravimetric analysis (TGA) curve for eugenol and ?-caryophyllene showed 100% weight loss in the range of 30°C-215°C whereas the weight loss of encapsulated eugenol and ?-caryophyllene occurred in the range of 300-580 °C, respectively.