Biochemistry And Microbial Sciences - Research Publications

Permanent URI for this collectionhttps://kr.cup.edu.in/handle/32116/27

Browse

Author: search.filters.author.Ramakrishna, WusirikaHas files: Yes

Search Results

Now showing 1 - 4 of 4
  • Thumbnail Image
    Item
    Nutrient enhancement of chickpea grown with plant growth promoting bacteria in local soil of Bathinda, Northwestern India
    (Springer, 2019) Dogra, Nitin; Yadav, Radheshyam; Kaur, Manpreet; Adhikary, Arindam; Kumar, Sanjeev; Ramakrishna, Wusirika
    Plant growth promoting bacteria (PGPB) enhance crop productivity as part of green technology to reduce the use of chemical fertilizers. They also have the capability to enhance macro- and micronutrient content of plants. In the present study, PGPB isolates belonging to Pseudomonas citronellis (PC), Pseudomonas sp. RA6, Serratia sp. S2, Serratia marcescens CDP13, and Frateuria aurantia (Symbion-K) were tested on two chickpea varieties, PBG1 and PBG5 grown for 30 days in local soil from Bathinda region in Northwestern India. PC and CDP13 were found to be better chickpea growth stimulators compared to the commercial Symbion-K based on shoot length and biomass. Most PGPB enhanced macro- and micronutrients in shoots to varying degrees compared to the control. PBG5 gave better response compared to PBG1 with reference to plant growth attributes and enhancement of the macronutrients, calcium, nitrogen and phosphorus and micronutrients, boron, copper, iron, and zinc. PBG5 is a high yielding variety with better resistance compared to PBG1. Overall, PGPB isolated from the local soil and PGPB from other parts of India were shown to be useful for enhancement of nutrient content and plant growth.
  • Thumbnail Image
    Item
    Metabolomics, biomass and lignocellulosic total sugars analysis in foxtail millet (Setaria italica) inoculated with different combinations of plant growth promoting bacteria and mycorrhiza
    (Communications in Plant Sciences, 2017) Dhawi, Faten; Datta, Rupali; Ramakrishna, Wusirika
    Foxtail millet (Setaria italica) is the second most widely produced millet with potential as a biofuel source. Employment of plant growth promoting bacteria (PGPB) and mycorrhiza could serve as environment-friendly alternatives for the use of excessive NPK fertilizers and producing biofuel. The highest increase of biomass was associated with endomycorrhiza combined with PGPB in comparison to control. Nuclear magnetic resonance (NMR) analysis detected 28 metabolites in foxtail shoot with most of them upregulated in ecto/endomycorrhiza group and combined with PGPB. The upregulation of metabolites associated with synthesis of amino acids correlated positively with biomass. The inoculation with both PGPB and endomycorrhiza gave the best results with reference to total sugar yield. Our study indicates that PGPB and endomycorrhiza combination is well suited for enhancing biomass and boosting sugar yield, which are useful attributes for utilizing foxtail millet as a biofuel source.
  • Thumbnail Image
    Item
    Proteomics provides insights into biological pathways altered by plant growth promoting bacteria and arbuscular mycorrhiza in sorghum grown in marginal soil
    (Elsevier B.V., 2017) Dhawi, Faten; Datta, Rupali; Ramakrishna, Wusirika; Dhawi, F.; Datta, R.; Ramakrishna, W.
    Sorghum is an economically important crop, a model system for gene discovery and a biofuel source. Sorghum seedlings were subjected to three microbial treatments, plant growth promoting bacteria (B), arbuscular mycorrhizal (AM) fungi mix with two Glomus species (G. aggregatum and G. etunicatum), Funelliformis mosseae and Rhizophagus irregularis (My), and B and My combined (My + B). Proteomic analysis was conducted followed by integration with metabolite, plant biomass and nutrient data. Out of 366 differentially expressed proteins in sorghum roots, 44 upregulated proteins overlapping among three treatment groups showed positive correlation with sorghum biomass or element uptake or both. Proteins upregulated only in B group include asparagine synthetase which showed negative correlation with biomass and uptake of elements. Phosphoribosyl amino imidazole succinocarboxamide protein with more than 50-fold change in My and My + B groups correlated positively with Ca, Cu, S and sucrose levels in roots. The B group showed the highest number of upregulated proteins among the three groups with negative correlation with sorghum biomass and element uptake. KEGG pathway analysis identified carbon fixation as the unique pathway associated with common upregulated proteins while biosynthesis of amino acids and fatty acid degradation were associated with common downregulated proteins. Protein-protein interaction analysis using STRING identified a major network with thirteen downregulated proteins. These findings suggest that plant-growth-promoting-bacteria alone or in combination with mycorrhiza enhanced radical scavenging system and increased levels of specific proteins thereby shifting the metabolism towards synthesis of carbohydrates resulting in sorghum biomass increase and uptake of nutrients. ? 2016
  • Thumbnail Image
    Item
    Mycorrhiza and heavy metal resistant bacteria enhance growth, nutrient uptake and alter metabolic profile of sorghum grown in marginal soil
    (Elsevier Ltd, 2016) Dhawi, Faten; Datta, Rupali; Ramakrishna, Wusirika; Dhawi, F.; Datta, R.; Ramakrishna, W.
    The main challenge for plants growing in nutrient poor, contaminated soil is biomass reduction, nutrient deficiency and presence of heavy metals. Our aim is to overcome these challenges using different microbial combinations in mining-impacted soil and focus on their physiological and biochemical impacts on a model plant system, which has multiple applications. In the current study, sorghum BTx623 seedlings grown in mining-impacted soil in greenhouse were subjected to plant growth promoting bacteria (PGPB or B) alone, PGPB with arbuscular mycorrhizal fungi (My), My alone and control group with no treatment. Root biomass and uptake of most of the elements showed significant increase in all treatment groups in comparison with control. Mycorrhiza group showed the best effect followed by My + B and B groups for uptake of majority of the elements by roots. On the contrary, biomass of both shoot and root was more influenced by B treatment than My + B and My treatments. Metabolomics identified compounds whose levels changed in roots of treatment groups significantly in comparison to control. Upregulation of stearic acid, sorbitol, sebacic acid and ferulic acid correlated positively with biomass and uptake of almost all elements. Two biochemical pathways, fatty acid biosynthesis and galactose metabolism, were regulated in all treatment groups. Three common pathways were upregulated only in My and My + B groups. Our results suggest that PGPB enhanced metabolic activities which resulted in increase in element uptake and sorghum root biomass whether accompanied with mycorrhiza or used solely. ? 2016 Elsevier Ltd.