Biochemistry And Microbial Sciences - Research Publications
Permanent URI for this collectionhttps://kr.cup.edu.in/handle/32116/27
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Item Genome-wide identification of NAC transcription factors in Avena sativa under salinity stress(Elsevier B.V., 2023-10-29T00:00:00) Bokolia, Muskan; Singh, Baljinder; Kumar, Avneesh; Goyal, Nandni; Singh, Kashmir; Chhabra, RavindreshBackground: NAC (NAM, ATAF1/2, and CUC2) is one of the most prominent family of plant-specific transcription factors that play diverse roles in plant growth and development as well as in abiotic stress responses in plants. The members of this family are recognized by presence of typical conserved NAC domain at the N-terminal and diverse C-terminal region. Results: In this study, we have identified 101 Avena sativa NAC (AsNAC) genes from the available Avena genome database. Genes were analyzed for their physicochemical properties, conserved motifs, gene structure, chromosomal localization, phylogenetic relationship, and cis-acting elements. The phylogenetic analysis illustrated that there were 15 subgroups in both Avena sativa and Arabidopsis thaliana. Mainly four types of cis-acting regulatory elements were present in the promoter regions of NAC genes, including hormone-responsive, light-responsive, stress-responsive and growth and developmental responsive elements. The chromosomal mapping analysis concluded that 101 NAC genes of Avena sativa were unevenly distributed on 21 chromosomes. Expression analysis identified 27 Avena NAC genes that respond to salt stress based on transcriptomic data analysis available on the NCBI SRA database. Significance: The genome-wide identification and molecular analysis of NAC TFs involved in environmental stress responses have the ability to overcome the limitations that came across in producing the transgenic crops with superior quality and improved production under abiotic stressed conditions. Future prospectives: These NAC genes may be considered as potential candidates for further explorations of functional analysis and could be used to develop stress tolerant lines in Avena sativa. � 2023 The Author(s)Item Critical review on uranium and arsenic content and their chemical mobilization in groundwater: A case study of the Malwa region Punjab, India(Elsevier B.V., 2023-05-03T00:00:00) Kumar, Vajinder; Maity, Arnab; Kumar, Avneesh; Saha, Sandip; Kay, Paul; Singh, Baljinder; Mukherjee, TirthaThe presence of pollutants like uranium and arsenic in the groundwater can have a terrible impact on people's health (both radiologically and toxicologically) and their economic conditions. Their infiltration into groundwater can occur through geochemical reactions, natural mineral deposits, mining and ore processing. Governments and scientists are working to address these issues, and significant progress has been achieved, but it's challenging to deal with and mitigate without adequately understanding the different chemical processes and the mobilization mechanism of these hazardous chemicals. Most of the articles and reviews have focused on the particular form of contaminants and specific sources of pollution, such as fertilizers. However, no literature report exists explaining why particular forms appear and the possible basis of their chemical origins. Hence, in this review, we tried to answer the various questions by devising a hypothetical model and chemical schematic flowcharts for the chemical mobilization of arsenic and uranium in groundwater. An effort has been made to explain how chemical seepage and excessive groundwater use resulted in the change in aquifers' chemistry, as evidenced by their physicochemical parameters and heavy metal analysis. Many technological advancements have taken place to mitigate these issues. Still, in low-middle-income countries, especially in the Malwa region of Punjab, also known as Punjab's cancer belt, paying a high amount for installing and maintaining these technologies is an unviable option. In addition to working to improve people's access to sanitary facilities and clean water to drink, the policy-level intervention would focus on increasing community awareness and continued research on developing better and more economical technologies. Our designed model/chemical flowcharts will help policymakers and researchers better understand the problems and alleviate their effects. Moreover, these models can be utilized in other parts of the globe where similar questions exist. This article emphasises the value of understanding the intricate issue of groundwater management through a multidisciplinary and interdepartmental approach. � 2023 Elsevier B.V.Item Bisphenol A in canned soft drinks, plastic-bottled water, and household water tank from Punjab, India(Elsevier B.V., 2022-11-19T00:00:00) Kumar, Ashwini; Singh, Daljit; Bhandari, Rajan; Malik, Ashok Kumar; Kaur, Sukhjeet; Singh, BaljinderThe aim of this study was the quantification of Bisphenol A (BPA) in canned soft drinks, plastic-bottled water and household water tanks from Punjab, India. Samples of five brands of canned soft drinks and plastic-bottled water purchased from local markets. Fifteen samples each of household plastic water tanks and concrete water tank were selected from three different cities (5 from each city) of Punjab, India. Samples were selected randomly. The plastic household water tank samples were collected in the months of December and June to check the effect of temperature on release of BPA in a plastic water tanks. The analysis method consists of microextraction in packed syringe (MEPS) followed by gas chromatography with mass spectrometric (GC-MS) detection. The detection limits and quantitation for BPA were 0.46 pg/mL and 1.52 pg/mL, respectively. Traces of BPA were detected in all canned soft drinks samples. Canned soft drinks and plastic-bottled water samples of five brands were having the BPA concentrations 8.2-14.01 pg/mL and 60-90 pg/mL, respectively. The BPA concentrations in the plastic water tanks were nondetected-12 pg/mL and nondetected-10 pg/mL in concrete water tank. � 2022 The Authors