Biochemistry And Microbial Sciences - Research Publications

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    Polydopamine films change their physicochemical and antimicrobial properties with a change in reaction conditions
    (Royal Society of Chemistry, 2018) Patel, Khushbu; Singh, Nimisha; Yadav, Jyoti; Nayak, Jyotsna M.; Sahoo, Suban K; Lata, Jeevan; Chand, Duni; Kumar, Shashank; Kumar, Rajender
    The morphology and physicochemical properties of polydopamine are not totally inherent and undergo changes with differing reaction conditions like the choice of solvent used for polymerization. The polymerisation of dopamine to polydopamine carried out in different solvents like sodium hydroxide, sodium bicarbonate, PBS and Tris leads to polydopamine with exceptionally different morphological and physicochemical features with each solvent. Additionally, the different physicochemical characteristics and morphologies bestow the polymer films with different extents of antimicrobial activity. Moreover, the findings supported by chemical evidence from X-ray photoelectron spectroscopy reveal that higher antibacterial activities were obtained against E. coli and S. aureus with polydopamine films prepared by Tris and NaOH solvent induced polymerization. The antibacterial activity observed in saline was found to be higher than that in PBS medium for both E. coli and S. aureus. The higher antibacterial activity of polydopamine films prepared in Tris and NaOH solvents was attributed to the covalent incorporation of -OH groups on the surface provided by nucleophilic Tris and NaOH solvents during the polymerisation process. The distinct physicochemical and morphological changes were supported by the results from contact angle measurements, FE-SEM, EDAX, AFM, and XPS analysis. The present finding provides insight into the different chemistry, morphologies and properties of the designed polydopamine films with controlled antibacterial/antifouling properties. Additionally, new insights into the mechanism of formation, physicochemical changes in morphology and properties of polydopamine coatings were revealed. ? the Owner Societies.
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    Characterization of WY 14,643 and its complex with Aldose reductase
    (Nature Publishing Group, 2016) Sawaya, M.R.; Verma, M.; Balendiran, V.; Rath, N.P.; Cascio, D.; Balendiran, G.K.
    The peroxisome proliferator, WY 14,643 exhibits a pure non-competitive inhibition pattern in the aldehyde reduction and in alcohol oxidation activities of human Aldose reductase (hAR). Fluorescence emission measurements of the equilibrium dissociation constants, Kd, of oxidized (hAR? NADP+) and reduced (hAR? NADPH) holoenzyme complexes display a 2-fold difference between them. Kd values for the dissociation of WY 14,643 from the oxidized (hAR? NADP+ ? WY 14,643) and reduced (hAR? NADPH? WY 14,643) ternary complexes are comparable to each other. The ternary complex structure of hAR? NADP+ ? WY 14,643 reveals the first structural evidence of a fibrate class drug binding to hAR. These observations demonstrate how fibrate molecules such as WY 14,643, besides being valued as agonists for PPAR, also inhibit hAR. ? The Author(s) 2016.