Biochemistry And Microbial Sciences - Master Dissertation

Permanent URI for this collectionhttps://kr.cup.edu.in/handle/32116/24

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20182

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    To Study the Alteration in Thymic Functionality in Experimental Visceral Leishmaniasis
    (Central University of Punjab, 2018) Mehara, Arjun Kumar; Jain,Manju
    Visceral Leishmaniasis (VL) is a neglected tropical disease and is potentially-fatal. Species belonging to L.donovani complex are the causative agent of VL in humans. Host immunity against VL critically depends on T cell based cell mediated immune response. VL is associated with lymphopenia such that progression of VL involves depletion of T cells. However, the origin and mechanism of T cell alterations in peripheral blood is not clearly understood. So we have tried to understand the origin of T cell changes in context of thymic functions which is the site of T cell development based on T cell Receptor Excision Circles analysis in experimental murine VL model. The result shows higher copy number of T cell Receptor Excision Circles (TRECs) in peripheral blood and a trend towards increase in developing thymocytes from thymi of infected mice compared to control uninfected group. The results imply that the peripheral T cell repertoires comprise a significant fraction of unexpanded naïve T cells. TREC change in thymocytes is statistically not significant implying no change in thymic output of naïve T cells. Thus our findings show that leishmania parasite can modulate T cell arm of immunity by altering the proliferation capacity of recent thymic naïve T cell emigrants.
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    Understanding Immune-etiology of Psoriasis: An autoimmune disease
    (Central University of Punjab, 2018) Kumari, Anjna; Jain, Manju
    Psoriasis is a chronic inflammatory autoimmune disease. Disease etiology is understood in terms of altered crosstalk between skin keratinocytes and immune cell infiltrates, specifically T cells leading to the development of characteristic psoriatic skin lesions. T cell alterations in skin lesions as well as in the peripheral blood of psoriatic patients have been shown to be associated with the disease condition. With a major research focus on keratinocyte abnormalities, more studies are required to understand the immune-etiology of disease. There are fewer reports with inconsistence findings on T cell changes in psoriatic patients. Towards this end, we performed a study using blood samples from psoriasis patients and healthy controls to access alterations in peripheral blood mononuclear cell and T cell count along with phenotyping of blood cells in terms of CD4+ Th and CD8+ Tc cells and expression pattern of T cell-associated cytokines in plasma samples. Furthermore, TREC analysis was done to understand possible origin of T cell alterations associated with psoriasis.