Botany - Research Publications
Permanent URI for this collectionhttps://kr.cup.edu.in/handle/32116/32
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Item Drought priming modulates ABF, GRFs, related microRNAs and induce metabolic adjustment during heat stress in chickpea(Elsevier Masson s.r.l., 2023-09-09T00:00:00) Juneja, Sumandeep; Saini, Rashmi; Mukit, Abdul; Kumar, SanjeevDrought and high temperature stress may occur concomitantly or individually in succession causing cellular dysfunctions. Abscisic acid (ABA) is a key stress regulator, and its responsive genes are controlled by ABRE (Abscisic acid Responsive Element)-binding factors (ABFs)and G-Box Regulatory factors (GRFs). Here, we identify ABFs, GRFs and targeting miRNAs in desi and kabuli chickpea. To validate their role after drought priming and subsequent high temperature stress, two contrasting chickpea varieties (PBG1 and PBG5) were primed and exposed to 32 �C, 35 �C and 38 �C for 12, 6 and 2 h respectively and analyzed for Physio-biochemical, expression of ABFs, GRFs and MiRNAs, and GC-MS based metabolite analysis. To ascertain the ABF-GRF protein-protein interactions, docking studies were carried out between the ABF3 and GRF14. Genome-wide analysis identified total 9 & 11 ABFs, and 11 GRFsin desi and kabuli respectively. Their gene structure, and motif composition were conserved in all subfamilies and only 10 and 12 genes have undergone duplication in both desi and kabuli chickpea respectively. These genes were differentially expressed in-silico. MiR172 and miR396 were identified to target ABFs and GRFs respectively. Protein-protein interaction (ABF3 and GRF14) might be successful only when the ABF3 was phosphorylated. Drought priming downregulated miR172 and miR396 and eventually upregulated targeting ABFs, and GRFs. Metabolite profiling (GC-MS) revealed the accumulation of 87 metabolites in Primed (P) and Non-Primed (NP) Chickpea plants. Tolerant cultivar (PBG5) responded better in all respects however both severity of stress and exposure are important factors and can produce broadly similar cellular response. � 2023 Elsevier Masson SASItem Drought priming evokes essential regulation of Hsp gene families, Hsfs and their related miRNAs and induces heat stress tolerance in chickpea(Elsevier B.V., 2023-07-26T00:00:00) Juneja, Sumandeep; Saini, Rashmi; Adhikary, Arindam; Yadav, Renu; Khan, Shahied Ahmed; Nayyar, Harsh; Kumar, SanjeevOptimum temperature is crucial for plant's survival. During high temperature stress, heat shock proteins (Hsps) are expressed many folds essentially controlled by explicit heat shock factors (Hsfs).We have narrowed key HSPs, related HSFs and miRNAs regulated after priming with drought stress and consequent heat stress in chickpea. Firstly, we identified Hsf and Hsp gene families in desi and kabuli chickpea using Genome-wide analysis. Thereafter, selected Hsfs, Hsps and related miRNAs were analyzed using qRT-PCR in contrasting chickpea varieties (PBG1 and PBG5) after drought priming and exposing at 32 �C 24 hrs, 35 �C 12 hrs, and 38 �C 6 hrs. An interaction network between Hsfs and Hsps was generated. 18 & 17 Hsfs and 42 & 34 Hsps were identified in the desi and kabuli, respectively. The gene structure and motif composition of the genes were found to be conserved in all subfamilies. A total of 32 heat shock genes were found to have undergone duplication. Most of the CaHsf and CaHsp genes were differentially expressed on exposure to a combination of drought priming and heat stress in both in-silico and qPCR analysis. Targeted miRNAs expression was coordinated with the respective genes. miR156, miR166, miR319, miR171, and miR5213 were identified to be targets of sHsps, Hsfs, and Hsps. The protein-protein interaction revealed that CaHsp18.2 and CaHsp70 might be controlled by CaHsfsA1. Drought priming strongly correlated with less membrane damage and better leaf water content. Higher harvest index and root shoot ratio significantly indicated effectiveness of priming and essential role of Hsf and Hsp and related miRNAs in heat stress tolerance. � 2023Item Drought priming modulates ABF, GRFs, related microRNAs and induce metabolic adjustment during heat stress in chickpea(Elsevier Masson s.r.l., 2023-09-09T00:00:00) Juneja, Sumandeep; Saini, Rashmi; Mukit, Abdul; Kumar, SanjeevDrought and high temperature stress may occur concomitantly or individually in succession causing cellular dysfunctions. Abscisic acid (ABA) is a key stress regulator, and its responsive genes are controlled by ABRE (Abscisic acid Responsive Element)-binding factors (ABFs)and G-Box Regulatory factors (GRFs). Here, we identify ABFs, GRFs and targeting miRNAs in desi and kabuli chickpea. To validate their role after drought priming and subsequent high temperature stress, two contrasting chickpea varieties (PBG1 and PBG5) were primed and exposed to 32 �C, 35 �C and 38 �C for 12, 6 and 2 h respectively and analyzed for Physio-biochemical, expression of ABFs, GRFs and MiRNAs, and GC-MS based metabolite analysis. To ascertain the ABF-GRF protein-protein interactions, docking studies were carried out between the ABF3 and GRF14. Genome-wide analysis identified total 9 & 11 ABFs, and 11 GRFsin desi and kabuli respectively. Their gene structure, and motif composition were conserved in all subfamilies and only 10 and 12 genes have undergone duplication in both desi and kabuli chickpea respectively. These genes were differentially expressed in-silico. MiR172 and miR396 were identified to target ABFs and GRFs respectively. Protein-protein interaction (ABF3 and GRF14) might be successful only when the ABF3 was phosphorylated. Drought priming downregulated miR172 and miR396 and eventually upregulated targeting ABFs, and GRFs. Metabolite profiling (GC-MS) revealed the accumulation of 87 metabolites in Primed (P) and Non-Primed (NP) Chickpea plants. Tolerant cultivar (PBG5) responded better in all respects however both severity of stress and exposure are important factors and can produce broadly similar cellular response. � 2023 Elsevier Masson SASItem Drought priming evokes essential regulation of Hsp gene families, Hsfs and their related miRNAs and induces heat stress tolerance in chickpea(Elsevier B.V., 2023-07-26T00:00:00) Juneja, Sumandeep; Saini, Rashmi; Adhikary, Arindam; Yadav, Renu; Khan, Shahied Ahmed; Nayyar, Harsh; Kumar, SanjeevOptimum temperature is crucial for plant's survival. During high temperature stress, heat shock proteins (Hsps) are expressed many folds essentially controlled by explicit heat shock factors (Hsfs).We have narrowed key HSPs, related HSFs and miRNAs regulated after priming with drought stress and consequent heat stress in chickpea. Firstly, we identified Hsf and Hsp gene families in desi and kabuli chickpea using Genome-wide analysis. Thereafter, selected Hsfs, Hsps and related miRNAs were analyzed using qRT-PCR in contrasting chickpea varieties (PBG1 and PBG5) after drought priming and exposing at 32 �C 24 hrs, 35 �C 12 hrs, and 38 �C 6 hrs. An interaction network between Hsfs and Hsps was generated. 18 & 17 Hsfs and 42 & 34 Hsps were identified in the desi and kabuli, respectively. The gene structure and motif composition of the genes were found to be conserved in all subfamilies. A total of 32 heat shock genes were found to have undergone duplication. Most of the CaHsf and CaHsp genes were differentially expressed on exposure to a combination of drought priming and heat stress in both in-silico and qPCR analysis. Targeted miRNAs expression was coordinated with the respective genes. miR156, miR166, miR319, miR171, and miR5213 were identified to be targets of sHsps, Hsfs, and Hsps. The protein-protein interaction revealed that CaHsp18.2 and CaHsp70 might be controlled by CaHsfsA1. Drought priming strongly correlated with less membrane damage and better leaf water content. Higher harvest index and root shoot ratio significantly indicated effectiveness of priming and essential role of Hsf and Hsp and related miRNAs in heat stress tolerance. � 2023