Botany - Research Publications

Permanent URI for this collectionhttps://kr.cup.edu.in/handle/32116/32

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Author: search.filters.author.Rani, PoojaHas files: Yes

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    DNA Barcoding, Phylogeny and Phylogeography of Green Sea Weed Ulva from Indian Subcontinent
    (Central University of Punjab, 2019) Rani, Pooja; Bast, Felix
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    Chloroplast DNA phylogeography of holy basil (Ocimum tenuiflorum) in Indian subcontinent
    (2014) Bast, Felix; Rani, Pooja; Meena, Devendra
    Ocimum tenuiflorum L., holy basil "Tulsi", is an important medicinal plant that is being grown and traditionally revered throughout Indian Subcontinent for thousands of years; however, DNA sequence-based genetic diversity of this aromatic herb is not yet known. In this report, we present our studies on the phylogeography of this species using trnL-trnF intergenic spacer of plastid genome as the DNA barcode for isolates from Indian subcontinent. Our pairwise distance analyses indicated that genetic heterogeneity of isolates remained quite low, with overall mean nucleotide p-distance of 5?10-4. However, our sensitive phylogenetic analysis using maximum likelihood framework was able to reveal subtle intraspecific molecular evolution of this species within the subcontinent. All isolates except that from North-Central India formed a distinct phylogenetic clade, notwithstanding low bootstrap support and collapse of the clade in Bayesian Inference. North-Central isolates occupied more basal position compared to other isolates, which is suggestive of its evolutionarily primitive status. Indian isolates formed a monophyletic and well-supported clade within O. tenuiflorum clade, which indicates a distinct haplotype. Given the vast geographical area of more than 3 million km 2 encompassing many exclusive biogeographical and ecological zones, relatively low rate of evolution of this herb at this locus in India is particularly interesting. ? 2014 Felix Bast et al.
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    DB IndAlgae: An on-line resource of marine algae from India identified on the basis of molecular and morphological features
    (Indian Academy of Sciences, 2016) Bhushan, Satej; Mehra, Richa; Rani, Pooja; Bast, Felix
    DbIndAlgae is a free on-line database of marine algae from India. It provides information about the geographical distribution, morphological characteristics and most importantly, sequence data of marine algae collected from different coasts. It is the only database which contains molecular data of the algal species from India. Identification of the species is based on both morphological as well as molecular information. The database also serves as an interface to the herbarium maintained at the Centre for Plant Sciences, Central University of Punjab, Bathinda. So far the database lists 45 marine algal species. Some algal species have been identified for the first time from India, but have already been reported from places other than India, whereas new species are those which have been identified by us for the first time as a part of this study and have never been reported elsewhere.
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    New record of Sargassum zhangii (Sargassaceae, Fucales) in India based on nuclear and mitochondrial DNA barcodes
    (Taylor and Francis Ltd., 2016) Bast, Felix; Bhushan, Satej; Rani, Pooja; John, Aijaz Ahmad
    Brown algal genus Sargassum encompasses some of the most invasive seaweeds in the oceans across the world. Here we report the occurrence of Sargassum zhangii in Palk Strait, southeast India. Hundreds of natural populations of this seaweed were observed in the collection site. BLASTn similarity search using nuclear DNA internal transcribed spacer1 (ITS1) and mitochondrial DNA cytochrome oxidase 1 (COX?1) gene of this isolate indicated Sargassum zhangii as the most homologous sequence available in the repository. Pairwise distances among these isolates were very low, 2.8?נ10?3 and 1.3?נ10?2 with ITS1 and COX?1, respectively, which suggests conspecificity and a recent introduction. Phylogenetic analyses along with other members of genus Sargassum conducted using Bayesian Inference resulted in well-resolved phylograms with robust clades comprising two isolates of S.?zhangii, further confirming conspecificity. With this first report of this seaweed outside China, invasive potential of S.?zhangii is highlighted that warrants immediate global attention. As this is the first time that molecular systematics have been used to catalogue species invasion in India, its efficacy, as well as the necessity for further cataloguing of species invasions using this approach, are highlighted. ? 2016 Dipartimento di Biologia, Universit? di Firenze.