Botany - Research Publications

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    Carrageenan based hydrogels and composites: A systematic investigation reporting their multidimentional essence
    (Nova Science Publishers, Inc., 2016) Ul Rehman, W.; Majeed, A.; Rani, P.; Saini, K.C.; Najar, R.A.; Mehra, R.; Singh, A.; Bast, Felix
    Carrageenans are red seaweed extracted sulfated linear polysaccharides which possess excellent gel forming properties. They are extensively used for their thickening, gel forming as well as stabilizing properties in food industries. Due to their good protein binding abilities, they are preferred in meat and dairy preservation. Structurally, they are polymers of alternately repeating disaccharide unit, ?-(133)-D-galactose and ?-(134)-3,6- anhydro-D- or L-galactose. Sulphation of the galactose gives them the distinguishing properties. Three isomeric forms of carrageenan with slightly different properties exist in nature. Their hydrogels are used in tissue engineering and controlled drug delivery while their composites find applications in wound dressings, protection of fish and meat from dehydration. In the field of medicine, carrageenans possess anticoagulant, antithrombotic, antiviral, and antitumor activities. Besides they also possess many industrial applications. ? 2016 Nova Science Publishers, Inc.
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    Morphological and molecular assessment of native carrageenophyte Hypnea valentiae (Cystocloniaceae, Gigartinales) in Indian Subcontinent
    (Phycological Society, 2014) Bast, Felix; Bhushan, Satej; John, Aijaz Ahmad
    Hypnea valentiae is an important red alga commercially cultivated in various parts of the world for the production of carrageenan. Presented in this report is findings of morphological and molecular observations of naturally-occurring populations of this alga collected from west and east coasts of India. Both the isolates had similar external as well as microscopic morphology. Nuclear ribosomal DNA Internal Transcribed Spacer-1 (ITS1) sequences from these geographical isolates had 4.35 x 10 -1 Tamura-3-Parameter (T3P) pairwise distance between them, which indicate significant evolutionary differences accumulated over time. In comparison, T3P distance between related genera Kappaphycus and Eucheuma was 1.85 x 10 -1 . In our phylogeny reconstruction using Bayesian Inference, both the isolates formed a well- supported clade along with the only available accession of this genus at ITS1 locus, indicating affiliation of both the isolates in this genus. Interestingly, isolate from the west coast was more basal in the phylogram, which suggests phylogenetically primitive position of this population. Newly generated DNA barcodes of the geographic isolates of this native carragenophyte in this study is expected to be a key in tracing its further dispersal routes, either natural or deliberate. This is the first report on the comparative morphological and molecular assessment of Hypnea from India.