Botany - Mphil Thesis

Permanent URI for this collectionhttps://kr.cup.edu.in/handle/32116/30

Browse

Filters

2013 - 20142

Settings

Subject: search.filters.subject.Phylogeography

Search Results

Now showing 1 - 2 of 2
  • Thumbnail Image
    Item
    Sequence-based phygeography and conservation of seaweeds from indian subcontinents
    (Central University of Punjab, 2014) Rani, Pooja; Bast, Felix
    Phylogeography is the study of historical events which are responsible for evolution and current distribution of a species in different geographical area. However very less record is available about marine macroalgae of Indian subcontinent. This study investigated the DNA barcoding and phylogeographical distribution of marine algae from the Indian subcontinent. Different algae samples collected from various coasts of Indian subcontinent are amplified using ITS, COX and rbcL primers. In our results, we found the occurrence of green algae like Ulva reticulata, Ulva intestinalis, Ulva fasciata, Ulva proliifera, Ulva ohnoi and one sample with Caulerpa scalpelliformis ; Red algae, Gracilaria foliifera, Gracilaria domingensis, Gracilaria corticata, Grateloupia Sp., Ceramium Sp., Centroceras clavulatum, Erythrocladia Sp., Erythrocladia irregularis, Acanthophora Sp., Dilsea socialis, Hypnea stelullifera, Sirodotia tenuissima and Dichotomaria Sp.; Brown algae, Sargassum zhangii, Sargassum megalocystum, Sargassum aquifolium and Turbinaria ornata in Indian subcontinent. Gracilaria domingenesis, Dilsea socialis, Sargassum megalosystum were first time reported in India. On the basis of molecular studies, we found that Ceramium Sp. Nov., Erythrocladia Sp. Nov., Acanthophora Sp Nov., Grateloupia Sp. Nov. and Dichotomaria Sp. Nov. were identified as new species. Erythrocladia irregularis was identified as endophytic algae inside green algae Cladophora glomerata. Phylogenetic tree was generated to analyse the evolutionary distance between different samples. Morphological and microscopic studies were performed for each sample. This study further helps in identification and documentation of new species and cryptic species. All samples were pressed for herbarium voucher.
  • Thumbnail Image
    Item
    DNA Barcoding and phylogography of brown seaweeds of coasts of indian subcontinent
    (Central University of Punjab, 2013) Bhushan, Satej; Bast, Felix
    Algae are one of the diverse groups of ubiquitous autotrophs. Their use as food was more or less initially confined to few East Asian and South American countries like China, Japan, Korea, Chile, Argentina and Brazil, but with the increased demand and limited supply for food combined with the medicinal properties of the marine macroalgae, they started getting the attention of policy makers and researchers alike all around the world. Brown seaweeds (Phaeophyceae) are mostly marine and characterized by presence of pigment fucoxanthin which gives them its coloured appearance. The present work aims to provide detailed molecular analysis of the brown seaweeds found in Indian coastal regions to study and characterize it taxonomically which has not been done till now in Indian context. Out of all the samples processed, one invasive species was detected, Sargassum zhangii, which is the first report of this algal species outside Chinese waters. The conspecificity was confirmed by a multi-faceted approach, including comparative morphology, microscopy, genetic distance analysis and computational phylogenetics using Maximum Likelihood and Bayesian Inference methods.