Human Genetics And Molecular Medicine - Research Publications

Permanent URI for this collectionhttps://kr.cup.edu.in/handle/32116/107

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Author: search.filters.author.Mishra, S

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    Synthesis of gadolinium oxide nanocuboids for in vitro bioimaging applications
    (Institute of Physics Publishing, 2019) Chawda, N; Mishra, S; Basu, M; Chander, H; Podder, R; Mahapatra, S.K; Banerjee, I.
    Undoped and Eu-doped gadolinium oxide (GGNCs and EGNCs) nanocuboids functionalized with D-gluconic acid (GA) were synthesised by a simple yet unique scheme using all the benign solvents and temperature. Samples were characterized and presented with properties like good dispersity, biocompatibility, and stability required for standard contrast agent used in magnetic resonance imaging (MRI) and optical coherence tomography (OCT). Biological assays such as 3-(4, 5-dimethyl-2-yl)-2, 5-diphenyltetrazoliumbromide (MTT) assay, flow cytometry and confocal microscopy were used to determine its biocompatibility, cellular internalization and optical cellular imaging using A549 and H1299 lung cancer cell lines. EGNCs treated with cell lines emitted red fluorescence which was used to track the internalization of EGNCs within the cells. GGNCs sample showed ?20% enhanced MRI relaxivity as compared to EGNCs; whereas EGNCs revealed better contrast in doctor scans of OCT. Samples could be used as promising candidate for other biomedical applications such as drug delivery when equipped with well functionalised drug molecules.
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    KIBRA Team Up with Partners to Promote Breast Cancer Metastasis
    (Springer, 2019) Singh G; Mishra, S; Chander, Harish
    Among women, breast cancer is the most frequently diagnosed cancer. Most of the breast cancers represent metastasis to distant organs at the time of diagnosis and accounts for the majority of deaths. Metastasis is characterized by many genetic aberrations including mutations, overexpression of oncogenes etc. KIBRA (KIdney/BRAin protein), a scaffolding protein is recently described as an important player in the process of invasion and metastasis. The Kidney/BRAin protein through its different domains interacts with various proteins to couple cytoskeleton arrangement, cell polarity and migration. N terminal and C terminal of the protein contains the WW, Internal C 2 & putative class III PDZ domain that interacts with DDR1, DLC1 & PKCζ. These protein-protein interactions equip the breast cancer cells to invade and metastasize. Here, we discuss a comprehensive knowledge about the KIBRA protein, its domains and the interacting partners involved in metastasis of breast cancer. © 2019, Arányi Lajos Foundation.