Human Genetics And Molecular Medicine - Research Publications

Permanent URI for this collectionhttps://kr.cup.edu.in/handle/32116/107

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Author: search.filters.author.Singh S.

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    Small regulatory molecules acting big in cancer: Potential role of mito-miRs in cancer
    (Bentham Science Publishers, 2019) Sharma P.; Bharat; Dogra N.; Singh S.
    MicroRNAs [miRNAs] are short, non-coding, single stranded RNA molecules regulating gene expression of their targets at the posttranscriptional level by either degrading mRNA or by inhibiting translation. Previously, miRNAs have been reported to be present inside the mitochondria and these miRNAs have been termed as mito-miRs. Origin of these mito-miRs may either be from mitochondrial genome or import from nucleus. The second class of mito-miRs makes it important to unravel the involvement of miRNAs in crosstalk between nucleus and mitochondria. Since miRNAs are involved in various physiological processes, their deregulation is often associated with disease progression, including cancer. The current review focuses on the involvement of miRNAs in different mitochondrial mediated processes. It also highlights the importance of exploring the interaction of miRNAs with mitochondrial genome, which may lead to the development of small regulatory RNA based therapeutic options.
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    Editorial: Novel biomarkers for type 2 diabetes
    (Frontiers Media S.A., 2019) Ahluwalia T.S.; Kilpel�inen T.O.; Singh S.; Rossing P.
    [No abstract available]
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    Evaluation of leaves of Goniothalamus wynaadensis Bedd. for inhibition of metabolic viability of cancer cells & antimicrobial efficacy
    (Elsevier GmbH, 2019) Sharma A.; Sharma P.; Singh S.; Karegoudar T.B.; Holla H.
    Introduction: Goniothalamus wynaadensis Bedd. is a less explored medicinal plant belonging to the family Annonaceae. This species has been traditionally used by tribes of Wayanad, Kerala, in India, and also Indonesian tribes for joint-related ailments and also as a deodorizer. The aim of the study was to analyze leaf extracts of the plant for their antimicrobial and anticancer properties along with phytochemical screening. Methods: MTT assay was used to assess the cytotoxic potential of plant extracts against MDA-MB-231 and A-549. Antimicrobial potential of plant extracts was analyzed via liquid broth turbidometry assay and well in agar plate methods. Identification tests were carried out for phytochemical screening. Results: Antimicrobial assay showed EC50 values for ethyl acetate extract 0.82 mg/mL against Escherichia coli, 0.82 mg/mL against Salmonella typhi, & 0.88 mg/mL against Staphylococcus aureus which are quite significant. Other solvent extracts also displayed antimicrobial activities at varying dosage. MTT assay was performed at the dose concentrations of 1 ?g/mL, 5 ?g/mL & 25 ?g/mL, 50 ?g/mL and 100 ?g/mL to confirm the cytotoxicity of the extracts. The ethyl acetate extract displayed EC50 values of 4.96 ?g/mL against A-549 lung cancer cell line and 2.50 ?g/mL against MDA-MB-231 breast cancer cell line. Other extracts were also found to be cytotoxic. Conclusion: Ethyl acetate & water extracts have demonstrated potent activity against Salmonella typhi, Escherichia coli, & Staphylococcus aureus bacteria as well as, showing cytotoxicity against tested cancer cell lines. These results can be explored to identify individual phyto-molecules from the extracts with anticancer & antimicrobial potential.