Administration

Permanent URI for this communityhttps://kr.cup.edu.in/handle/32116/1

Browse

Author: search.filters.author.Kohli, R.K.Subject: search.filters.subject.Phytotoxicity

Search Results

Now showing 1 - 2 of 2
  • No Thumbnail Available
    Item
    Phytotoxicity and cytotoxicity of Citrus aurantiifolia essential oil and its major constituents: Limonene and citral
    (Elsevier B.V., 2017) Fagodia, S.K.; Singh, H.P.; Batish, Daizy R.; Kohli, R.K.
    The essential oils are fast emerging as the source of natural herbicides owing to their environmentally benign properties. The focus of the present study, thus, was to investigate the phytotoxicity and cytotoxicity of Citrus aurantiifolia oil, and its major constituents-citral and limonene. C. aurantiifolia oil was selected due to its extreme commercialisation and safe nature. GC?MS analysis revealed that C. aurantiifolia oil is rich in monoterpenes (83.93%), with limonene (40.92%) and citral (27.46%) as the major compounds. Phytotoxicity was assessed against three agricultural weeds, Avena fatua, Echinochloa crus-galli and Phalaris minor, at concentration ranging from 0.10?1.50?mg/ml. Percent germination, IC50 value and seedling growth (root and coleoptile length) were significantly reduced in a dose-response manner. C. aurantiifolia oil, citral and limonene caused alteration in the cell cycle of Allium cepa root meristematic cells as evidenced by decrease in mitotic index (MI) and increase in chromosomal aberrations at progressive concentrations (0.01?0.10?mg/ml) and time periods (3?h and 24?h). Cytotoxic evaluation confirmed mitodepressive effect of the tested volatiles though the intensity was variable. Overall, citral was the most toxic followed by C. aurantiifolia oil and limonene. The significant phytotoxic activity of C. aurantiifolia oil and citral suggests the possibility of being developed into eco-friendly and acceptable products for weed management in agriculture system. ? 2017 Elsevier B.V.
  • Thumbnail Image
    Item
    Exogenous Nitric Oxide (NO) interferes with lead (Pb)-induced toxicity by detoxifying reactive oxygen species in hydroponically grown wheat (Triticum aestivum) roots
    (Public Library of Science, 2015) Kaur, Gurpreet; Singh, Harminder Pal; Daizy R. Batish; Mahajan, Priyanka; Kohli, R.K.; Valbha, Rishi
    Nitric Oxide (NO) is a bioactive signaling molecule that mediates a variety of biotic and abiotic stresses. The present study investigated the role of NO (as SNP [sodium nitroprusside]) in ameliorating lead (Pb)-toxicity in Triticum aestivum (wheat) roots. Pb (50 and 250 ?M) alone and in combination with SNP (100 ?M) was given to hydroponically grown wheat roots for a period of 0-8 h. NO supplementation reduced the accumulation of oxidative stress markers (malondialdehyde, conjugated dienes, hydroxyl ions and superoxide anion) and decreased the antioxidant enzyme activity in wheat roots particularly up to 6 h, thereby suggesting its role as an antioxidant. NO ameliorated Pb-induced membrane damage in wheat roots as evidenced by decreased ion-leakage and in situ histochemical localization. Pb-exposure significantly decreased in vivo NO level. The study concludes that exogenous NO partially ameliorates Pb-toxicity, but could not restore the plant growth on prolonged Pb-exposure. ? 2015 Kaur et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.