Department Of Biochemistry And Microbial Sciences

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Author: search.filters.author.Prajapati, Kumari SunitaSubject: search.filters.subject.Dual therapy

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    Synthesis of thiolated chlorogenic acid-capped silver nanoparticles for the effective dual action towards antimicrobial and anticancer therapy
    (Springer Science and Business Media Deutschland GmbH, 2022-07-22T00:00:00) Nayak, Jyotsnamayee; Prajapati, Kumari Sunita; Kumar, Shashank; Sahoo, Suban K.; Kumar, Rajender
    Chlorogenic acid (CGA) was thiolated using Traut�s reagent. Then, thiolated chlorogenic acid (CAT) functionalized on the silver nanoparticles (AgNPs). The resulting CAT-functionalized silver nanoparticles (CAT-AgNPs) have higher stability with a uniform spherical shape compared to naked AgNPs. The average particle size is 10�nm, as revealed by transmission electron microscopy (TEM). The antimicrobial activity was higher against both gram-positive and gram-negative bacteria of spherically stable nanoparticles evaluated by the disk diffusion method. Minimal inhibitory concentration (MIC) values for CAT-AgNPs were 130��g/ml for Staphylococcus aureus and 150��g/ml for Escherichia coli. Cytotoxicity studies showed that the CAT-AgNPs show maximum activity against MCF-07 cell lines with an IC50 value of 20�ng/ml. Compared to traditional noncovalent interactions, the Ag�S covalent linkage of CAT with AgNPs helped in highly stable capping. The prepared CAT-AgNPs show anticancer and antibacterial activities, thus providing an efficient antimicrobial nano-drug carrier. Nanoparticles with a dual-mode of action help in preventing fouling of nanocarriers while delivering drugs to targeted cells, opening up many applications in nanobiotechnology and nanomedicine. � 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.