Department Of Biochemistry And Microbial Sciences

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Author: search.filters.author.Ramakrishna, W.Subject: search.filters.subject.Plant growth promoting bacteria

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    Bacteria from native soil in combination with arbuscular mycorrhizal fungi augment wheat yield and biofortification
    (Elsevier, 2020) Yadav, R; Ror, P; Rathore, P; Ramakrishna, W.
    Plant growth promoting bacteria (PGPB) have been used to enhance crop productivity. The effect of native PGPB and arbuscular mycorrhizal (AM) fungi in combination on wheat yield, biofortification and soil enzymatic activity is a relatively unexplored area. Twenty seven bacterial isolates from three different soils were characterized for their plant growth promoting traits. A total of three native and five non-native bacteria were used with and without arbuscular mycorrhizal (AM) fungi in an open greenhouse pot experiment with two wheat varieties to evaluate their effect on wheat yield, nutrient uptake, and soil health parameters. Wheat plants subjected to native PGPB (CP4) (Bacillus subtilis) and AM fungi treatment gave the best results with reference to macronutrient (nitrogen and phosphorus), micronutrient (iron and zinc) content in wheat grains and yield-related parameters, including thousand grain weight, number of grains per spike and total tillers per plant in both wheat cultivars. Treatment with CP4 and CP4 plus AM fungi enhanced total chlorophyll in wheat leaves indicating higher photosynthetic activity. Significant improvement in soil health-related parameters, including soil organic matter and dehydrogenase activity, was observed. Significant correlation among grain yield-related parameters, nutrient enhancement, and soil health parameters was observed in PGPB and AM fungi treated plants, especially HD-3086. These results provide a roadmap for utilizing native PGPB and AM fungi for enhancing wheat production in Punjab state of India and exploring their utility in other parts of the country with different soil and environmental conditions. - 2020 Elsevier Masson SAS
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    Proteomics provides insights into biological pathways altered by plant growth promoting bacteria and arbuscular mycorrhiza in sorghum grown in marginal soil
    (Elsevier B.V., 2017) Dhawi, Faten; Datta, Rupali; Ramakrishna, Wusirika; Dhawi, F.; Datta, R.; Ramakrishna, W.
    Sorghum is an economically important crop, a model system for gene discovery and a biofuel source. Sorghum seedlings were subjected to three microbial treatments, plant growth promoting bacteria (B), arbuscular mycorrhizal (AM) fungi mix with two Glomus species (G. aggregatum and G. etunicatum), Funelliformis mosseae and Rhizophagus irregularis (My), and B and My combined (My + B). Proteomic analysis was conducted followed by integration with metabolite, plant biomass and nutrient data. Out of 366 differentially expressed proteins in sorghum roots, 44 upregulated proteins overlapping among three treatment groups showed positive correlation with sorghum biomass or element uptake or both. Proteins upregulated only in B group include asparagine synthetase which showed negative correlation with biomass and uptake of elements. Phosphoribosyl amino imidazole succinocarboxamide protein with more than 50-fold change in My and My + B groups correlated positively with Ca, Cu, S and sucrose levels in roots. The B group showed the highest number of upregulated proteins among the three groups with negative correlation with sorghum biomass and element uptake. KEGG pathway analysis identified carbon fixation as the unique pathway associated with common upregulated proteins while biosynthesis of amino acids and fatty acid degradation were associated with common downregulated proteins. Protein-protein interaction analysis using STRING identified a major network with thirteen downregulated proteins. These findings suggest that plant-growth-promoting-bacteria alone or in combination with mycorrhiza enhanced radical scavenging system and increased levels of specific proteins thereby shifting the metabolism towards synthesis of carbohydrates resulting in sorghum biomass increase and uptake of nutrients. ? 2016