Department Of Biochemistry And Microbial Sciences

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Subject: search.filters.subject.Carbohydrate metabolism

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    Pseudomonas citronellolis alleviates arsenic toxicity and maintains cellular homeostasis in chickpea (Cicer arietinum L.)
    (Elsevier Masson s.r.l., 2022-05-19T00:00:00) Adhikary, Arindam; Saini, Rashmi; Kumar, Rashpal; Singh, Inderjit; Ramakrishna, Wusirika; Kumar, Sanjeev
    Arsenic is a hazardous metalloid that causes detrimental effects on plant growth and metabolism. Plants accumulate arsenic in edible parts that consequently enter the food chain leading to many health problems. Metal tolerant plant growth-promoting bacteria (PGPB) ameliorate heavy metal toxicity. In this study, the effect of arsenic (As5+) and the role of PGPB Pseudomonas citronellolis (PC) in mitigating As5+ toxicity and associated metabolic alterations in chickpea were assessed. Five chickpea varieties (PBG1, GPF2, PDG3, PDG4 and PBG5) were evaluated for arsenic accumulation, translocation, and its interference with metabolic and defense processes. As5+ (40 mg kg?1) interfered with plant metabolism and enhanced the antioxidative and carbohydrate metabolizing enzyme's activity but PC treatment maintained the activity at par with control. PC also facilitated the accumulation of As5+ in the root system and restricted its translocation to the shoot. Further, to map the metabolic changes, Gas chromatography Mass Spectroscopy (GC-MS) based metabolite profiling and gene expression analysis (qRT-PCR) were performed in the best and worst-performing chickpea varieties (PBG1 and PBG5). 48 metabolites of various metabolic pathways (amino acid, carbohydrate, and fatty acid) were altered in As5+ and PC treatment. Gene expressions showed correlation with biochemical analysis of the antioxidative enzymes and carbohydrate metabolizing enzymes while PC treatment improved chlorophyll biosynthesis enzyme CaDALA expression in As5+ treated plants. Therefore, PC mitigates As5+ toxicity by restricting it in the roots thereby maintaining the cellular homeostasis under As5+ stress in chickpeas. � 2022 Elsevier Masson SAS
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    In Silico Identification of Novel Natural Inhibitors Of Carbohydrate Metabolic Pathway In Cancer Cells
    (Central University of Punjab, 2018) Dash, Swastika; Kumar, Shashank
    Carbohydrate metabolism in cancer cells is linked to the 'Warburg Effect' which states that, under aerobic conditions, cancer cells metabolize approximately ten fold more glucose to lactate in a given time than normal cells; typically altered glycolytic pathway regulation. This has made the blocking of glycolytic pathway enzymes, a fascinating strategy to find treatment for cancer. This project addresses in a comprehensive manner the main glycolytic enzymes accounting for high-rate glycolysis in cancer cells. In addition, highlights of inhibitors that can be used to target the particular enzymes to decrease proliferation have also been done. Furthermore, besides the known inhibitors, receptor-based molecular docking of certain methylated flavonoids was performed with the proteins (isozymes of carbohydrate metabolic pathway enzymes) to find the lead inhibitors. The proteins used in the study are GLUT1 (4PYP), Hexokinase2 (2NZT), Phosphofructokinase2 (2AXN), Pyruvate kinaseM2 (3GQY), Lactate dehydrogenase A (4AJP) and Enolase2 (5IDZ). The dock scores were in the range of -5.88 to -9.68 against different target proteins. The methylated flavonoids 2-(3,4-dihydroxyphenyl)-3,5-dihydroxy-7-methoxy-4H-chromen- 4-one, 5,7-dihydroxy-2-(3-hydroxy-4-methoxyphenyl)-6,8-dimethoxy-4H-chromen-4- one, 2-(3,4-dimethylphenyl)-5,7-dimethyl-4H-chromen-4-one and 6-hydroxy-3,5,7,8- tetramethoxy-2-(3,4,5-trimethoxyphenyl)-4H-chromen-4-one showed better dock scores for the target proteins in comparison to the standard inhibitors. Thus these methylated flavonoids might be considered promising leads for further development of glycolytic pathway inhibitors in cancer cells.