School Of Health Sciences

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    KIBRA Team Up with Partners to Promote Breast Cancer Metastasis
    (Springer, 2019) Singh G; Mishra, S; Chander, Harish
    Among women, breast cancer is the most frequently diagnosed cancer. Most of the breast cancers represent metastasis to distant organs at the time of diagnosis and accounts for the majority of deaths. Metastasis is characterized by many genetic aberrations including mutations, overexpression of oncogenes etc. KIBRA (KIdney/BRAin protein), a scaffolding protein is recently described as an important player in the process of invasion and metastasis. The Kidney/BRAin protein through its different domains interacts with various proteins to couple cytoskeleton arrangement, cell polarity and migration. N terminal and C terminal of the protein contains the WW, Internal C 2 & putative class III PDZ domain that interacts with DDR1, DLC1 & PKCζ. These protein-protein interactions equip the breast cancer cells to invade and metastasize. Here, we discuss a comprehensive knowledge about the KIBRA protein, its domains and the interacting partners involved in metastasis of breast cancer. © 2019, Arányi Lajos Foundation.
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    Expression of KIBRA in Breast Cancer Cell Lines
    (Central University of Punjab, 2018) Singh, Garima; Chander,Harish
    Breast cancer the most frequently diagnosed cancer and its metastasis to distant organs accounts the majority of deaths. Numerous genes and proteins are involved in the cause of metastasis. Though KIBRA is one of the component of Hippo Pathway and is reported as tumor suppressor but this scaffolding protein has also been found to be an emerging and important player in the process of metastasis. It has been reported that KIBRA protein interacts with various proteins through its domain and leads cytoskeleton arrangement, cell polarity and migration. N terminal and C terminal of the protein contains the WW, Internal C2 & putative class III PDZ domain that interacts with DDR1, DLC1 & PKC? and helps the breast cancer cells to metastasize. To study whether KIBRA is involved in breast cancer metastasis, we checked its expression at both protein and mRNA level by Immunoblotting and Real Time PCR which showed increased KIBRA expression in ER positive cells. Further investigation to elucidate the role of KIBRA in ER positive cells, ER transfection and immunoblotting in triple negative breast cancer cell lines were performed, which indicated that ER leads to enhanced KIBRA expression in breast cancer cells.