Department Of Zoology

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Author: search.filters.author.Sarkar, DebarshiSubject: search.filters.subject.epididymis

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    Ontogeny of TR?1 expression in the mouse testis and epididymis during postnatal development
    (John Wiley and Sons Inc, 2022-06-27T00:00:00) Sarkar, Debarshi; Jaiswal, Asmita; Singh, Shio Kumar
    Thyroid hormone (T3) acts on the testis via thyroid hormone receptor alpha 1 (TR?1), though the cellular localization of TR?1 in testis remains controversial. Studies on the presence of TR?1 in the epididymis are also lacking. The present study, therefore, examined the cellular localization and expression pattern of TR?1 in testis and epididymis of Parkes mice during postnatal development. Immunohistochemical results showed localization of TR?1 in interstitial and tubular compartments of the testis all through the development. On postnatal day (PND) 14, only leptotene spermatocytes showed TR?1-immunoreactivity in the testis, while at PND 28, 42, and 90, a diverse staining pattern for TR?1 was seen in almost all the seminiferous tubules mainly in leptotene spermatocytes, round and elongating spermatids, and in Leydig cells. Further, qRT-PCR and immunoblot analyses showed that TR?1 was expressed in the testis at the transcript as well as protein level throughout the postnatal development. TR?1 was also seen in principal cells of the epididymis, with maximal expression at PND 90. TR?1 was also present in cauda epididymidal spermatozoa of adult mice at PND 90. The results suggest that TR?1 is expressed in the testis and epididymis and that it may help to regulate the spermatogenic process and male fertility. � 2022 Wiley-VCH GmbH.
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    Effect of Trigonella foenum-graecum L. seed extract on the reproductive system of male mice and possible mechanism of its action on spermatogenesis
    (John Wiley and Sons Inc, 2022-04-13T00:00:00) Singh, Akanksha; Sarkar, Debarshi; Singh, Shio Kumar
    Fenugreek seed exhibits antidiabetic, antineoplastic, hepatoprotective, antidepressant and immunomodulatory properties. Fenugreek also causes antifertility effects in rodents. However, the impact of fenugreek seed on male reproduction and the possible mode of its action are not properly evaluated. Herein, we examined the effect of aqueous seed extract of fenugreek (FSE) and the possible mechanism of its action on male reproductive health in mice. Parkes mice were orally administered FSE (600�mg/kg body weight/day) or distilled water for 28 and 56�days, respectively. Various sperm parameters, histopathology, serum testosterone level and fertility indices were assessed. Furthermore, steroidogenic enzymes activities, oxidative status and germ cell dynamics in the testis were evaluated. Toxicological endpoints were also assessed. Treatment with FSE caused degenerative changes in the testis histoarchitecture. The treatment also affected various sperm parameters and concentrations of sialic acid and fructose in the epididymis and seminal vesicle, respectively. Fenugreek treatment also had negative impact on oxidative status and germ cell dynamics in the testis; fertility indices were also affected in female mice impregnated by the extract-treated male mice, though libido of the treated male mice remained unaffected. Results show that treatment with FSE caused adverse effects on the male reproductive health and pregnancy outcome in Parkes mice. � 2022 Wiley-VCH GmbH.