Department Of Environmental Science And Technology

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Author: search.filters.author.Dhanya, M.S.Subject: search.filters.subject.Biodegradation

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    Chlorpyrifos degrading potential of a Bacterial isolate from Thermal Power Plant Soil
    (World Research Association, 2023-01-27T00:00:00) Dubey, Sheetal; Dhanya, M.S.
    The pesticides had a significant role in crop protection and in agricultural production. The organophosphate pesticides are used against many crop pests. But the injudicious application results in residues of organophosphate pesticides in soil routed to food chain by different means and affect the human health. There is a great need for remediation of the toxic residues from the environment. The microbial bioremediation is a safe and eco-friendly technique for pesticide removal. The present study focused on the degradation ability of a bacterium previously isolated from the soil contaminated with fly ash from coal based thermal power plant. The 16S rRNA sequencing confirmed the bacterium as Lysinibacillus fusiformis. The chlorpyrifos utilization capability of the isolated bacterium was studied in mineral salt medium supplemented with 50 mg L-1 chlorpyrifos as sole carbon source at 30oC and 120 rpm for 15 days. The bacterium was able to grow in the medium with the production of alkaline phosphatase and reduction in pH of the growth media. The bacterium degraded 16.54 percent of chlorpyrifos in 15 days. The chlorpyrifos utilization for bacterial biomass production confirmed the in situ biodegradation ability of the bacterium. � 2023 World Research Association. All rights reserved.
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    Evaluation of Biodegradation Efficiency of Xylene Pretreated Polyethylene Wastes by Isolated Lysinibacillus fusiformis
    (Technoscience Publications, 2022-09-04T00:00:00) Kalia, Arun; Dhanya, M.S.
    The ability of the bacterial degradation of low-density polyethylene (LDPE) waste by Lysinibacillus fusiformis isolated from hydrocarbon-contaminated soil was investigated in the present study. The potential of the bacterial isolate to utilize LDPE waste bags of two different thicknesses in a month as a sole carbon source in mineral salt media was assessed. Further, the effect of pretreatment by xylene on the bacterial degradation of LDPE waste bags (0.5 percent w/v) in 30 days was investigated. The isolated Lysinibacillus fusiformis was able to degrade 9.51 percent of LDPE with 30 ?m thickness but able to degrade only 1.45 percent of LDPE having 50 ?m thickness. The bacterial biomass was 1.77 times higher on LDPE- 30 ?m containing media in comparison to LDPE- 50 ?m. The xylene pretreatment of LDPE wastes enhanced the biodegradation efficiency of isolated Lysinibacillus fusiformis to 12.09 and 1.97 percent respectively in 30 ?m and 50 ?m thick LDPE bags. The xylene pre-treatment improved the bacterial growth on media with LDPE of both thicknesses. The adherence of bacterium on the surface of LDPE was found more on 50 ?m thick xylene treated LDPE compared to its untreated LDPE than 30 ?m thick LDPE films. The xylene pre-treatment of polyethylene waste had an additive effect on the biodegradation of waste LDPE films with a significant effect on thickness. � 2022 Technoscience Publications. All rights reserved.