Department Of Environmental Science And Technology
Permanent URI for this communityhttps://kr.cup.edu.in/handle/32116/84
Browse
2 results
Search Results
Item Chlorpyrifos degrading potential of a Bacterial isolate from Thermal Power Plant Soil(World Research Association, 2023-01-27T00:00:00) Dubey, Sheetal; Dhanya, M.S.The pesticides had a significant role in crop protection and in agricultural production. The organophosphate pesticides are used against many crop pests. But the injudicious application results in residues of organophosphate pesticides in soil routed to food chain by different means and affect the human health. There is a great need for remediation of the toxic residues from the environment. The microbial bioremediation is a safe and eco-friendly technique for pesticide removal. The present study focused on the degradation ability of a bacterium previously isolated from the soil contaminated with fly ash from coal based thermal power plant. The 16S rRNA sequencing confirmed the bacterium as Lysinibacillus fusiformis. The chlorpyrifos utilization capability of the isolated bacterium was studied in mineral salt medium supplemented with 50 mg L-1 chlorpyrifos as sole carbon source at 30oC and 120 rpm for 15 days. The bacterium was able to grow in the medium with the production of alkaline phosphatase and reduction in pH of the growth media. The bacterium degraded 16.54 percent of chlorpyrifos in 15 days. The chlorpyrifos utilization for bacterial biomass production confirmed the in situ biodegradation ability of the bacterium. � 2023 World Research Association. All rights reserved.Item Chlorpyrifos Degradation in Semi-Arid Soil by Pseudomonas fluorescens Strain CD5 Isolated from Manured Soil(Taylor and Francis Ltd., 2022-07-19T00:00:00) Dubey, S.; Dhanya, M.S.The present paper focused on investigating the potential of indigenous Pseudomonas fluorescens strain CD5 isolated from manure-enriched soil as a bioremediation agent for chlorpyrifos biodegradation in semi-arid soil of Bathinda district of Punjab, India. The soil had sandy loam texture with slightly alkaline pH and low organic matter content of 0.36%. The growth pattern, alkaline phosphatase enzyme production and chlorpyrifos utilization capability of Pseudomonas fluorescens strain CD5 were screened in mineral salt medium and the results revealed degradation of 78.19% of 500 mg L?1 chlorpyrifos within 15�days. The pattern of chlorpyrifos degradation was further studied in natural conditions of semi-arid soil by the Pseudomonas fluorescens strain CD5 and photolysis by sunlight for a period of 105�days. The biodegradation of chlorpyrifos at 500 mg kg?1 sterile soil by Pseudomonas fluorescens strain CD5 was 59.68% in comparison to 26% of photodegradation and enhancing total degradation. The residual chlorpyrifos in sterile soil after biodegradation by Pseudomonas fluorescens strain CD5 under natural light condition was 14.33%. The alkaline phosphatase enzyme activity in soil was found positively correlated with the chlorpyrifos degradation. The Pseudomonas fluorescens strain CD5 can be an effective candidate for the bioremediation of chlorpyrifos-contaminated soil. � 2022 Taylor & Francis.