Department Of Environmental Science And Technology
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Item Optimized extraction, composition, antioxidant and antimicrobial activities of exo and intracellular polysaccharides from submerged culture of Cordyceps cicadae(BioMed Central Ltd., 2015) Sharma, S.K.; Gautam, N.; Atri, N.S.Background: Cordyceps cicadae is known as Jin Chan Hua in Traditional Chinese Medicine and known to possess different pharmacological activities. Presently, it was collected from the wild and isolated. Mycelial culture was optimized for extraction of polysaccharides under submerged culture conditions. Besides antioxidant, antibacterial activities of extracted polysaccharides were tested for first time. Methods: Exo-polysaccharides (EPS) and intracellular polysaccharides (IPS) production was tested under different factors (medium capacity, rotation speed, pH, incubation time, temperature, carbon, nitrogen, minerals sources and carbon to nitrogen ratio) by orthogonal experiments using one-factor-at-a-time method. Monosaccharides composition of polysaccharides produced by C. cicadae was determined using high performance liquid chromatography. Antioxidant and antimicrobial activities on eight bacterial strains were checked by different standard procedures. Results: Factors viz., medium capacity, rotation speed, incubation time, pH and temperature affected the EPS and IPS production under submerged culture conditions. EPS and IPS production was observed to vary with different carbon and nitrogen sources as well as C/N ratio. Glucose was the major component of polysaccharides (63.10 ? 4.15 %). Extracted EPS and IPS showed higher antioxidant potential with significant DPPH radical scavenging activity, ABTS radical scavenging activity, reducing power and iron chelating activity. Antimicrobial activities of EPS and IPS varied among the tested bacterial strains. IPS showed slightly higher inhibition rate to all the tested bacterial strains as compared to EPS. Maximum inhibition zones of IPS (12.9 ? 0.2 mm) and EPS (12.5 ? 0.3 mm) was observed against Pseudomonas aeruginosa at 10 % con. However, both EPS and IPS fractions showed broad spectrum for all the pathogenic microbial strains tested. The MIC of both the extracts ranged from 60-100 mg/mL. Conclusions: EPS and IPS production from submerged culture of C. cicadae with significant antioxidant and antibacterial potential can be enhanced with the combination of several factors which can be used for large scale industrial fermentation of C. cicadae. ? 2015 Sharma et al.Item Metacordyceps dhauladharensis sp. nov., a new entomopathogenic fungus from India(Turkiye Klinikleri Journal of Medical Sciences, 2015) Sharma, S.K.; Gautam, N.This paper describes a new species of Clavicipitaceae fungi belonging to the genus Metacordyceps G.H. Sung, J.M. Sung, Hywel-Jones & Spatafora, defined as Metacordyceps dhauladharensis sp. nov., from India. The diagnostic features of this new species are stipitate stromata of brownish color and an elongated fertile head on a Hymenopteran host. The perithecium size of this species was measured as 152?257 ? 65?72 ?m, and the asci were measured as 30?48 ?m in length and 2.5 ?m in breadth. Based on molecular studies, the sequence of 469 bp of this species showed only 86% homology with Metacordyceps indigotica (Kobayasi & Shimizu) Kepler, G.H. Sung & Spatafora. ? T?B?TAK.Item Chemical and Bioactive Profiling, and Biological Activities of Coral Fungi from Northwestern Himalayas(Nature Publishing Group, 2017) Sharma, S.K.; Gautam, N.Ramaria Fr. and Clavaria L. are the two major genera of coral mushrooms within families Gomphaceae and Clavariaceae, respectively. Besides having important role in forest ecology, some species of these are reported to possess high nutraceutical and bioactive potential. There is a hidden diversity of coral mushrooms in Northwestern Himalayas. Present studies describe the detailed biochemical profiling and antioxidant, and antibacterial activities of twelve coral mushroom species. Biochemical profiling of nutrients and nutraceuticals was done with standard techniques and by using HPLC, UPLC and GC. Experiments were also conducted to check the toxic metals detection. Antioxidant activities were calculated using EC50 values from mushroom extracts. Antibacterial activities were checked on six pathogenic bacterial strains through minimum inhibition concenterations. Although, differences were observed in the net values of individual species but all the species were found to be rich in protein, macro and micro minerals, carbohydrates, unsaturated fatty acids, essential amino acids, phenolics, tocopherols, anthocynadins and carotenoids. All the species showed significant antioxidant and antibacterial activities. These species are reported to free from heavy toxic metals. Present studies will open the way for their large scale commercial exploitations and use in pharmaceutical industries as antioxidant, antibacterial and nutraceutical constituents. ? 2017 The Author(s).Item Chemical, Bioactive, and Antioxidant Potential of Twenty Wild Culinary Mushroom Species(Hindawi Limited, 2015) Sharma, S.K.; Gautam, N.The chemical, bioactive, and antioxidant potential of twenty wild culinary mushroom species being consumed by the people of northern Himalayan regions has been evaluated for the first time in the present study. Nutrients analyzed include protein, crude fat, fibres, carbohydrates, and monosaccharides. Besides, preliminary study on the detection of toxic compounds was done on these species. Bioactive compounds evaluated are fatty acids, amino acids, tocopherol content, carotenoids (?-carotene, lycopene), flavonoids, ascorbic acid, and anthocyanidins. Fruitbodies extract of all the species was tested for different types of antioxidant assays. Although differences were observed in the net values of individual species all the species were found to be rich in protein, and carbohydrates and low in fat. Glucose was found to be the major monosaccharide. Predominance of UFA (65-70%) over SFA (30-35%) was observed in all the species with considerable amounts of other bioactive compounds. All the species showed higher effectiveness for antioxidant capacities. ? 2015 S. K. Sharma and N. Gautam.