Department Of Environmental Science And Technology

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Author: search.filters.author.Kalia, Arun

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    Effect of Used Engine Oil and UV-Thermal Pretreatments on Biodegradation of Low-Density Polyethylene by Lysinibacillus fusiformis TPB
    (National Institute of Science Communication and Policy Research, 2022-08-31T00:00:00) Kalia, Arun; Dhanya, M.S.
    The present study focused on the impact of Used Engine Oil (UEO) and abiotic pretreatments by ultraviolet (UV) radiation and thermal treatment at 70�C for 144 h on the potential of Lysinibacillus fusiformis TPB isolated from hydrocarbon contaminated soil for the biodegradation of low-density polyethylene (LDPE) in mineral salt medium at 30�C and 150 rpm for 30 days. The isolated L. fusiformis TPB degraded 9.51% of LPDE films without any treatment and used as the sole carbon source for biomass production. The supplementation of used engine oil (0.5% v/v) enhanced biodegradation of untreated LDPE films to 11.96% comparable to a non-ionic surfactant Tween 80. The abiotic pretreatments had also facilitated metabolism of LDPE by L. fusiformis TPB. The biodegradation of UV treated LDPE by L. fusiformis TPB was 13.78% and was significantly higher than thermally treated LDPE with 12.89% biodegradation. The Fourier Transform Infrared spectrum revealed structural and morphological changes in the LDPE films by abiotic pretreatments and were associated with addition of carbonyl groups and change in double bond index. The Scanning Electron Microscopy analysis of LDPE films from UEO and UV-thermal pretreated LDPE supplemented mineral salt media confirmed the improved bacterial colonization and biofilm formation. The isolated L. fusiformis TPB had LDPE degradation potential and biodegradation had improved by UEO supplementation and UV-thermal pretreatments. � 2022 Scientific Publishers. All rights reserved.
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    Evaluation of Biodegradation Efficiency of Xylene Pretreated Polyethylene Wastes by Isolated Lysinibacillus fusiformis
    (Technoscience Publications, 2022-09-04T00:00:00) Kalia, Arun; Dhanya, M.S.
    The ability of the bacterial degradation of low-density polyethylene (LDPE) waste by Lysinibacillus fusiformis isolated from hydrocarbon-contaminated soil was investigated in the present study. The potential of the bacterial isolate to utilize LDPE waste bags of two different thicknesses in a month as a sole carbon source in mineral salt media was assessed. Further, the effect of pretreatment by xylene on the bacterial degradation of LDPE waste bags (0.5 percent w/v) in 30 days was investigated. The isolated Lysinibacillus fusiformis was able to degrade 9.51 percent of LDPE with 30 ?m thickness but able to degrade only 1.45 percent of LDPE having 50 ?m thickness. The bacterial biomass was 1.77 times higher on LDPE- 30 ?m containing media in comparison to LDPE- 50 ?m. The xylene pretreatment of LDPE wastes enhanced the biodegradation efficiency of isolated Lysinibacillus fusiformis to 12.09 and 1.97 percent respectively in 30 ?m and 50 ?m thick LDPE bags. The xylene pre-treatment improved the bacterial growth on media with LDPE of both thicknesses. The adherence of bacterium on the surface of LDPE was found more on 50 ?m thick xylene treated LDPE compared to its untreated LDPE than 30 ?m thick LDPE films. The xylene pre-treatment of polyethylene waste had an additive effect on the biodegradation of waste LDPE films with a significant effect on thickness. � 2022 Technoscience Publications. All rights reserved.
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    Recent advancements in hydrocarbon bioremediation and future challenges: a review
    (Springer Science and Business Media Deutschland GmbH, 2022-05-23T00:00:00) Kalia, Arun; Sharma, Samriti; Semor, Nisha; Babele, Piyoosh Kumar; Sagar, Shweta; Bhatia, Ravi Kant; Walia, Abhishek
    Petrochemicals are important hydrocarbons, which are one of the major concerns when accidently escaped into the environment. On one hand, these cause soil and fresh water pollution on land due to their seepage and leakage from automobile and petrochemical industries. On the other hand, oil spills occur during the transport of crude oil or refined petroleum products in the oceans around the world.�These hydrocarbon and petrochemical spills have not only posed a hazard to the environment and marine life, but also linked to numerous ailments like cancers and neural disorders. Therefore, it is very important to remove or degrade these pollutants before their hazardous effects deteriorate the environment. There are varieties of mechanical and chemical methods for removing hydrocarbons from polluted areas, but they are all ineffective and expensive. Bioremediation techniques provide an economical and eco-friendly mechanism for removing petrochemical and hydrocarbon residues from the affected sites. Bioremediation refers to the complete mineralization or transformation of complex organic pollutants into the simplest compounds by biological agents such as bacteria, fungi, etc. Many indigenous microbes present in nature are capable of detoxification of various hydrocarbons and their contaminants. This review presents an updated overview of recent advancements in various technologies used in the degradation and bioremediation of petroleum hydrocarbons, providing useful insights to manage such problems in an eco-friendly manner. � 2022, King Abdulaziz City for Science and Technology.