Department Of Environmental Science And Technology

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Author: search.filters.author.Kaur, Gurpreet

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    ASSOCIATION OF OXIDATIVE STRESS WITH CYP1A2, CYP3A4, CYP2B6 AND PON1 GENETIC POLYMORPHISM IN AGRICULTURAL COMMUNITY OF BATHINDA
    (Central University of Punjab, 2018) Kaur, Gurpreet; Jain, A. K. and Singh, Sandeep
    The aim of present study is to evaluate the association of oxidative stress with CYP1A2, CYP3A4, CYP2B6 and PON1 genetic variation in the pesticide exposed (occupationally) population. The present study demonstrated that the relative risk (RR) of declined antioxidant defense (Glutathione, Catalase, Superoxide Dismutase, Glutathione peroxidases, Glutathione Reductase) mechanism was significantly more in occupationally pesticide exposed group (n=120) as compared to unexposed group (n=84) from Bathinda district of Punjab (India). Although the age, BMI and alcohol intake were observed to be independent risk factors to decline the antioxidant defense mechanism, but the pesticide exposure was observed to be another risk factor to increase the oxidative stress inside the body. The gas chromatographic analysis revealed the residues of organophosphates (chlorpyriphos, dichlorvos, ethoprophos) and herbicides (atrazine, butachlor, alachlor, metolachlor) in the blood samples of population under investigation. Our results showed a dose dependent decrease in the cell viability following pesticide treatment (detected in blood samples) alone or in combination in hPBMC and A549 human lung cancer cell line. The pesticide treatment resulted the cell death in apoptotic manner via increased reactive oxygen species and cell cycle arrest at G0/G1 phase of cell cycle. Genetic variation analysis revealed the detection of two missense mutations [g.40991390G>T (SNP ID: rs33926104), g.40991388T>C (unknown)] on CYP2B6 gene of chromosome 19 in the coding region (exon 1) that results in the R29S and D28G amino acid change in CYP2B6 protein. One missense mutation (g.99758180C>T) has been detected in CYP3A4 gene that leads to change of amino acid from valine to isoleucine (V489I) at 489 location of protein. The mutated proteins have been predicted to change the structure and conformation of protein structure, thereby can alter its interaction with various DNA, membrane, ligand or . g.99757990A>T, g.99758050A>C and g.99758022A>T had been detected in CYP3A4 gene that may impair the post-translational modification of protein. So, the oxidative stress and pesticide residue accumulation inside the body could be the reason for more health problems in Bathinda district. Further, there is need to elaborate the research to study the genetic polymorphism in all pesticide metabolism genes.
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    Inhibitory effect of essential oil of ocimum sanctum l. against pesticide induced genotoxicity
    (Central University of Punjab, 2012) Kaur, Gurpreet; Mittal, Sunil
    The increase in pesticide contamination in the environment is of great concern due to its strong association with genetic material. The plants are considered as natural drugs to decline such toxic effects. In present study, the genotoxicity of Monocrotophos has been evaluated in lung cancer cell line i.e. A549 and H1299. The further step is to evaluate the prevention Monocrotophos induced genotoxic effect by pretreatment and post-treatment of O. sanctum essential oil. The results of present study suggest that eugenol (46%) and caryophyllene (28%) are the major components of essential oil of O. sanctum apart from other minor components. The essential oil cause increase in cell proliferation rate at lower concentration (20 ?g/ml for 6hrs.) in A549 cells while decrease in cell proliferation rate with increase in essential oil treatment after 20 ?g/ml concentration. Also, the cell viability has not been affected at 10 ?g/ml concentration in both the cell lines. On the other hand, Monocrotophos also cause increase cell proliferation rate at lower concentration (6.25 ?M - 200 ?M) for 6 hrs. in A549 cells and significant decrease in cell proliferation rate at higher concentration (1 mM -50 mM) in both the cell line. The results suggest that the H1299 cells, lacking p53 expression, are more sensitive to essential oil and Monocrotophos concentrations. The genotoxic study revealed that the Monocrotophos caused increase in comet length, % DNA in tail, tail length, tail intensity and decrease in Head intensity but pre-treat and post-treatment show protective action with respect to various Monocrotophos concentrations. It has been observed that post-treatment is better in comparison to pretreatment of Ocimum sanctum essential oil in all studied parameters. It can be concluded that the essential oils help the cells to compensate the oxidative stress generated inside the cell and hence can protect them from pesticide induced genotoxic effect.
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    Fruit waste (peel) as bio-reductant to synthesize silver nanoparticles with antimicrobial, antioxidant and cytotoxic activities
    (University of South Bohemia, 2018) Annu; Ahmed, Shakeel; Kaur, Gurpreet; Sharma, Praveen; Singh, Sandeep; Ikram, Saiqa
    Since last decade, biogenic synthesis of metal or metal-oxide nanoparticles is emerging as an alternative method, which is environment friendly, simple and safe to use. In this article, fruit waste (peel) extract (FWE) of three citrus fruits viz. Citrus limon, Citrus sinensis, and Citrus limetta were used as bio-reductant for green and sustainable synthesis of silver nanoparticles (AgNPs). As-synthesised AgNPs were characterized by using UV-vis spectroscopy, Dynamic light scattering, and High Resolution Transmission Electron Microscopy. TEM studies revealed 9-46 nm size range of synthesized AgNPs. The antimicrobial and antioxidant activities were also studied by using Agar well diffusion method and DPPH Assay, respectively. Nanoparticles showed good antimicrobial activity against both Gram positive (S. aureus) and Gram negative (E. coli) bacteria. Further, bioactivity assays revealed selective cytotoxicity (anticancer) of the nanoparticles against human lung cancer cell line A549. The nanoparticles are able to induce cancer cell specific apoptosis at G0/G1 phase of cell cycle. The results showed potential mechanism of action of nanoparticles via augmentation of antioxidant system in cancer cells. Over all, this study show multifaceted potential bioactivities of nanoparticles generated from fruit waste. ? 2018 Faculty of Health and Social Sciences, University of South Bohemia in Ceske Budejovice.
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    Eradicating Cancer Stem Cells: Concepts, Issues, and Challenges
    (Springer New York LLC, 2018) Kaur, Gurpreet; Sharma, Praveen; Dogra, Nilambra; Singh, Sandeep
    The cells of malignant cancers result in the evolution of cells with stem-like characteristics, commonly known as cancer stem cells (CSCs). Progress of anticancer therapies is severely hampered because of disease relapse mostly in a more aggressive form due to CSCs. These CSCs are more or less like embryonic or tissue stem cells, known for their capacity of self-renewal, exactly recapitulate of the original tumor. Deregulation of key stem cell pathways like Wnt, Hedgehog (Hh), and Notch is attributed towards the rise of CSCs. Recent breakthroughs offer better insights into CSC signaling. Scientists have developed several combinatorial therapies like targeting one/multiple of these CSC pathways. The article summarized various markers used to identify CSCs and discuss major signaling pathways in them. The futuristic probabilities to use CSC therapeutics in clinical development have been discussed. Our views have been highlighted on the future directions for targeting advances in the clinical development. ? 2018, Springer Science+Business Media, LLC, part of Springer Nature.