Department Of Environmental Science And Technology

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Author: search.filters.author.Singh, Jatinder

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    Isolation of indigenous butachlor (Herbicide) degrading bacteria from the agricultural fields of punjab
    (Central University of Punjab, 2014) Singh, Jatinder; Yogalakshmi, K.N.
    Butachlor (N-Butoxymethyl-2-chloro-2', 6'-diethyl acetanilide) is a chloroacetalanilide herbicide widely used in paddy fields. Due to extensive application, high stability and persistence in soil and water, has resulted in its ubiquitous presence as a pollutant. Hence, it becomes necessary to degrade the herbicide through an eco friendly and cost effective method. In this study, the degradation of butachlor herbicide was studied under controlled laboratory condition. The soil was collected from the agricultural fields of Punjab. The inoculum from the soil was enriched in 0.16mmol/L of butachlor at 130 rpm and 30 ?C. The enriched culture was plated and the best growing bacteria was isolated and designated as JF. The biodegradation of strain JF was evaluated at 0.16 and 0.32mmol/L concentration of butachlor. The isolate showed a degradation efficiency of 91.87% and 78.08% at 0.16mmol/L and 0.32mmol/L of butachlor, respectively in 120 hours (pH 7). The bacterial isolate JF was identified as Staphylococcus sp. through biochemical characterization. This study highlights the first report of isolation of butachlor degrading bacteria from the native soil of Punjab.
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    Comparative Analysis of Laccase Immobilization on Magnetic Iron Nanoparticles using Two Activating Agents:EDAC and Cyanuric Chloride
    (Tamil Nadu Scientific Research Organization (TNSRO), 2016) Das, Anamika; Singh, Jatinder; Yogalakshmi, K. N.
    Surface modification improves the covalent bonding of enzymes onto the magnetic nanoparticles. The present study aims to evaluate the effect of surface activators (EDAC (1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide) and Cyanuric chloride) in immobilization of enzymes. Nanoparticles prepared by co-precipitation method ranged insize from 15-20 nm. The nanoparticles possessed crystalline property as confirmed by the XRD (X-ray powder diffraction) peaks. SEM-EDS (Scanning Electron Microscopy- Energy Dispersive X-ray Spectroscopy) analysis of EDAC and cyanuric acid activated nanoparticles showed atomic sulphur percent of 0.08% and 0.02%, respectively. It is concluded that EDACwas more successful in loading more enzymes than cyanuric acid. Bradford estimation of the unbound protein after first wash for ENP-EDAC and ENP-CC was 29.1 μg/mL and 132.1 μg/mL, respectively. EDAC is a potential surface modifier for enzyme immobilization process.
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    Copper(II) Bioremoval by a Rhizosphere Bacterium, Stenotrophomonas acidaminiphila MYS1-Process Optimization by RSM Using Box–Behnken Design
    (Springer, 2017) Manohari; Singh, Jatinder; Yogalakshmi, K. N.
    A copper-tolerant bacterium strain MYS1 was isolated from Brass effluent-contaminated Cynodon dactylon rhizosphere and identified as Stenotrophomonas acidaminipila by 16S rRNA analysis. Bacterium culture was enriched in copper(II) supplemented nutrient broth. Bacterial potential for copper(II) bioremoval was investigated under optimized parameters. Three parameters—pH, temperature and copper(II) concentration—were optimized through response surface methodology (RSM). Box–Behnken design (BBD) with quadratic model was selected. Seventeen experimental runs were carried out to get the desired response. Model’s significance was confirmed by high R2 value (0.9941), low P value (<0.0001) and F value (131.32). Effect of different parameters on bioremoval of copper(II) was determined by response contour and surface graphs. Results showed that optimum values for copper(II) removal were obtained at pH (5.0), temperature (32.5 °C) and copper(II) concentration (250 mg/L). Under these optimized conditions, maximum bacterium growth (2.87 µg/mg) and copper(II) bioremoval (94.1%) were demonstrated after 120 and 168 h of incubation, respectively. High percentage of copper(II) removal at such a higher concentration confirmed the feasibility of bacterium Stenotrophomonas acidaminiphila MYS1 in copper bioremediation and industrial effluent treatment.