Department Of Environmental Science And Technology

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    Optimization of Copper (II) Removal by Response Surface Methodology Using Root Nodule Endophytic Bacteria Isolated from Vigna unguiculata
    (Springer International Publishing, 2016) Manohari, R.; Yogalakshmi, K.N.
    The present study was conducted to investigate copper tolerance and bioremediation potential in endophytic bacteria isolated from Vigna unguiculata root nodules. Total ten endophytes were isolated on yeast mannitol agar and enriched in copper (II) sulfate (CuSO4) up to 500?mg/L. Four endophytes belonging to genera Bacillus and Arthrobacter showed copper tolerance. The isolates were identified as Arthrobacter tumbae MYR1, Bacillus safensis MYR2, Bacillus pumilus MYR3 and Bacillus sp. MYR4 using 16S ribosomal RNA (rRNA) analysis. Response surface methodology was used for copper (II) removal optimization. The model was significant with R2, P and F value of 0.9780, <0.0001, and 34.54, respectively. Results showed that highest copper (II) bioremoval of 82.8?% was obtained at pH 5.0, temperature 32.5??C, and 600?mg/L copper concentration after 168?h of incubation. The isolates were tested for plant growth promotion and all the strains produced indole acetic acid (IAA) and showed 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity. The study concludes that endophytic bacteria possessed greater potential for copper tolerance and bioremediation. ? 2016, Springer International Publishing Switzerland.
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    Treatment of seafood processing wastewater using upflow microbial fuel cell for power generation and identification of bacterial community in anodic biofilm
    (Academic Press, 2016) Jayashree, C.; Tamilarasan, K.; Rajkumar, M.; Arulazhagan, P.; Yogalakshmi, K.N.; Srikanth, M.; Banu, J.R.
    Tubular upflow microbial fuel cell (MFC) utilizing sea food processing wastewater was evaluated for wastewater treatment efficiency and power generation. At an organic loading rate (OLR) of 0.6 g d-1, the MFC accomplished total and soluble chemical oxygen demand (COD) removal of 83 and 95%, respectively. A maximum power density of 105 mW m-2 (2.21 W m-3) was achieved at an OLR of 2.57 g d-1. The predominant bacterial communities of anode biofilm were identified as RB1A (LC035455), RB1B (LC035456), RB1C (LC035457) and RB1E (LC035458). All the four strains belonged to genera Stenotrophomonas. The results of the study reaffirms that the seafood processing wastewater can be treated in an upflow MFC for simultaneous power generation and wastewater treatment. ? 2016 Elsevier Ltd.