School Of Environment And Earth Sciences

Permanent URI for this communityhttps://kr.cup.edu.in/handle/32116/83

Browse

Subject: search.filters.subject.Antioxidant enzymes

Search Results

Now showing 1 - 2 of 2
  • No Thumbnail Available
    Item
    A cross-sectional study to correlate antioxidant enzymes, oxidative stress and inflammation with prevalence of hypertension
    (Elsevier Inc., 2022-10-28T00:00:00) Kaur, Sukhchain; Rubal; Kaur, Satveer; Kaur, Amandeep; Kaur, Sandeep; Gupta, Sushil; Mittal, Sunil; Dhiman, Monisha
    Aims: Hypertension a multifactorial consequence of environmental factors, life style and genetics is the well-recognized risk factor contributing to coronary heart diseases. The antioxidant imbalance, excessive reactive oxygen species (ROS) leads to oxidative stress which is pivotal in progression of hypertension. The present study aims to understand the complex interaction between oxidative stress, inflammation and antioxidant system which is crucial to maintain cellular homeostasis which further can exaggerate hypertension pathophysiology. Materials and methods: The metabolic profile of hypertensive and normotensive subjects from Malwa region, Punjab was compared by estimating lipid profile, cardiac, hepatic and renal markers. The oxidative stress markers (protein carbonyls and lipid peroxidation), inflammatory markers (Nitric oxide, Myeloperoxidase and advanced oxygen protein products), and antioxidant enzymes (Superoxide Dismutase, Catalase, and Total Antioxidant Capacity) were analyzed. Key findings: It is observed that the metabolic markers are altered in hypertensive subjects which further these subjects showed increased oxidative, inflammatory profile and compromised antioxidant status when compared with normotensive subjects. Co-relation analysis validated the involvement of inflammation and oxidative stress in impaired endothelial function and vital organ damage. Significance of study: These markers may act as early indicators of hypertension which usually do not show any physical symptoms, thus can be diagnosed and treated at the earliest. The current study suggests that disturbed homeostasis, a consequence of altered interaction between antioxidant system and inflammatory events raises the oxidative stress levels which eventually leads to hypertension and associated complications. These indicators can serve as early indicators of future chronic complications of hypertension. � 2022
  • No Thumbnail Available
    Item
    ASSOCIATION OF OXIDATIVE STRESS WITH CYP1A2, CYP3A4, CYP2B6 AND PON1 GENETIC POLYMORPHISM IN AGRICULTURAL COMMUNITY OF BATHINDA
    (Central University of Punjab, 2018) Kaur, Gurpreet; Jain, A. K. and Singh, Sandeep
    The aim of present study is to evaluate the association of oxidative stress with CYP1A2, CYP3A4, CYP2B6 and PON1 genetic variation in the pesticide exposed (occupationally) population. The present study demonstrated that the relative risk (RR) of declined antioxidant defense (Glutathione, Catalase, Superoxide Dismutase, Glutathione peroxidases, Glutathione Reductase) mechanism was significantly more in occupationally pesticide exposed group (n=120) as compared to unexposed group (n=84) from Bathinda district of Punjab (India). Although the age, BMI and alcohol intake were observed to be independent risk factors to decline the antioxidant defense mechanism, but the pesticide exposure was observed to be another risk factor to increase the oxidative stress inside the body. The gas chromatographic analysis revealed the residues of organophosphates (chlorpyriphos, dichlorvos, ethoprophos) and herbicides (atrazine, butachlor, alachlor, metolachlor) in the blood samples of population under investigation. Our results showed a dose dependent decrease in the cell viability following pesticide treatment (detected in blood samples) alone or in combination in hPBMC and A549 human lung cancer cell line. The pesticide treatment resulted the cell death in apoptotic manner via increased reactive oxygen species and cell cycle arrest at G0/G1 phase of cell cycle. Genetic variation analysis revealed the detection of two missense mutations [g.40991390G>T (SNP ID: rs33926104), g.40991388T>C (unknown)] on CYP2B6 gene of chromosome 19 in the coding region (exon 1) that results in the R29S and D28G amino acid change in CYP2B6 protein. One missense mutation (g.99758180C>T) has been detected in CYP3A4 gene that leads to change of amino acid from valine to isoleucine (V489I) at 489 location of protein. The mutated proteins have been predicted to change the structure and conformation of protein structure, thereby can alter its interaction with various DNA, membrane, ligand or . g.99757990A>T, g.99758050A>C and g.99758022A>T had been detected in CYP3A4 gene that may impair the post-translational modification of protein. So, the oxidative stress and pesticide residue accumulation inside the body could be the reason for more health problems in Bathinda district. Further, there is need to elaborate the research to study the genetic polymorphism in all pesticide metabolism genes.