School Of Basic And Applied Sciences

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Author: search.filters.author.Jairath, AnkurHas files: Yes

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    Status of endogenous antioxidants defense in preconditioned contrasting chickpea in precondioned contrasting chickpea (cicer arietinum) seedlingls
    (Central University of Punjab, 2014) Jairath, Ankur; Kumar, Sanjeev
    Plants experience various abiotic stresses of low or elevated temperature, drought and heavy metals. Chickpea (CicerarietinumL.) is one of the important crop belongs to the family Leguminosae and it is the second most important grain legume plant in the world after common bean. Chickpea has evolved as a spring crop in warmer climate of West Asia. Therefore, it is prone to chilling injury as in north India; chickpea is cultivated in winter season. To protect the plant from chilling injury, preconditioning the plants with mild drought stress might triggers the signalling pathways that will prepare the plants to combat lethal cold stress. This is determined by monitoring the various cell responses at different phases. Therefore antioxidative enzyme activity of the SOD, APX, GPOX, LOX, CAT enzymes was done. In our study, both the varieties, PDG3 and GPF2 showed equalvariation towards SOD, CAT, GPOX, and APX activity. Enhanced SOD, CAT & GPOX activity in both the varieties showed increased tolerance towards cold stress. Less LOX activity showed preconditioning decreased membrane damage in preconditioned seedlings. Thus, preconditioning with mild drought stress has enhanced tolerance capacity of the plant during the cold stress.
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    Alternate mild drought stress (20.1 MPa PEG) immunizes sensitive chickpea cultivar against lethal chilling by accentuating the defense mechanisms
    (Springer, 2016) Kaur, Simranjeet; Jairath, Ankur; Singh, Inderjeet; Nayyar, Harsh; Kumar, Sanjeev
    The changes in climate particularly, the rise in temperature and humidity affect the physiological functions of plants subsequently affecting crop productivity adversely. A strategy is required which can be directly implemented in fields to induce the tolerance in crop plants. In present study, two chickpea varieties with contrasting sensitivity PDG3 (Tolerant) and GPF2 (Sensitive) were raised hydroponically, preconditioned with mild drought stress (0.1 MPa PEG-6000) for 3 days (above 0.1 MPa is lethal) and subsequently recovered for double time (6 days) and finally exposed to lethal cold stress (4 °C) for 3 days. We hypothesize that preconditioning with non-lethal drought stress may immunize the plants to combat lethal cold stress. Membrane integrity improved in root and shoot, lipid peroxidation decreased to control level in preconditioned seedlings. Cellular respiration ability (% TTC reduction) increased in the preconditioned seedlings to almost 90 % in the shoot and 60 % in the root, concurrently it was 45 % in non-preconditioned seedlings. Proline content also increased in preconditioned seedlings, especially roots. Carbohydrate had a shift in terms of a high amount of total, reducing sugars and starch in non-preconditioned seedlings compared to preconditioned. Both PDG3 and GPF2 showed enhanced SOD, CAT and GPOX activity indicating tolerance against cold-induced oxidative stress and preconditioning induced improvement against lethal cold stress.