School Of Basic And Applied Sciences

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Author: search.filters.author.Kumar V.Has files: Yes

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    Comparative analysis of metabolites in contrasting chickpea cultivars
    (Springer, 2019) Ghosh A.; Dadhich A.; Bhardwaj P.; Babu J.N.; Kumar V.
    Chickpea (Cicer arietinum L.) is a good source of nutrients for animals and human consumption. In the present study, we analyzed the anthocyanin and total phenolic contents in two contrasting (desi and kabuli) chickpea cultivars. The quantification of anthocyanins showed higher amount in desi as compared to kabuli chickpea. The total phenolic contents was estimated in desi and kabuli chickpea using two different solvents (50% Acetone and 70% Methanol extracts) for coverage of all potential phenolic compounds. In continuation, desi chickpea culitvars (himchana and ICC4958) were found to be significantly higher total phenolic contents (in both solvent extracts) as compared to kabuli cultivars (JGK-03 and L-552). Higher phenolic contents was found to be directly correlated to higher anthocyanin contents in desi as compared to kabuli chickpea. The volatile organic compounds were also analyzed using gas chromatography mass spectroscopy technique in both cultivars. The significant compositional differences in volatile organic composition (polar and non-polar) of desi and kabuli cultivars were also found to be noticed using two different solvent extractions (methanol and chloroform). The comparative analysis of volatile organic acids in methanolic and chloroform extracts of desi cultivars (himchana and ICC4958), kabuli cultivars (JGK-03 and L-552) and between desi and kabuli cultivars was also carried out for in-depth understanding of the differential patterns of low molecular weight metabolites. Six metabolites were found to be common in all four selected cultivars in chloroform extracted samples, while four were found to be common in all four selected cultivars in methanolic extracted samples. The remaining detected metabolites are uncommon among different cultivars and represented as cultivar specific signatory metabolites. In conclusion, the present investigation revealed higher anthocyanin and phenolic contents in desi cultivars as compared to kabuli cultivars and differential accumulation of volatile organic compounds in chickpea cultivars. The metabolite alterations among desi and chickpea cultivars could be the potential attribute for diversity, resilience and commercial usuages.
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    PPInS: a repository of protein-protein interaction sitesbase
    (Nature Publishing Group, 2018) Kumar V.; Mahato S.; Munshi, Anjana; Kulharia, Mahesh
    Protein-Protein Interaction Sitesbase (PPInS), a high-performance database of protein-protein interacting interfaces, is presented. The atomic level information of the molecular interaction happening amongst various protein chains in protein-protein complexes (as reported in the Protein Data Bank [PDB]) together with their evolutionary information in Structural Classification of Proteins (SCOPe release 2.06), is made available in PPInS. Total 32468 PDB files representing X-ray crystallized multimeric protein-protein complexes with structural resolution better than 2.5 Å had been shortlisted to demarcate the protein-protein interaction interfaces (PPIIs). A total of 111857 PPIIs with ~32.24 million atomic contact pairs (ACPs) were generated and made available on a web server for on-site analysis and downloading purpose. All these PPIIs and protein-protein interacting patches (PPIPs) involved in them, were also analyzed in terms of a number of residues contributing in patch formation, their hydrophobic nature, amount of surface area they contributed in binding, and their homo and heterodimeric nature, to describe the diversity of information covered in PPInS. It was observed that 42.37% of total PPIPs were made up of 6-20 interacting residues, 53.08% PPIPs had interface area ?1000 Å 2 in PPII formation, 82.64% PPIPs were reported with hydrophobicity score of ?10, and 73.26% PPIPs were homologous to each other with the sequence similarity score ranging from 75-100%. A subset ''Non-Redundant Database (NRDB)'' of the PPInS containing 2265 PPIIs, with over 1.8 million ACPs corresponding to the 1931 protein-protein complexes (PDBs), was also designed by removing structural redundancies at the level of SCOP superfamily (SCOP release 1.75). The web interface of the PPInS (http://www.cup.edu.in:99/ppins/home.php) offers an easy-to-navigate, intuitive and user-friendly environment, and can be accessed by providing PDB ID, SCOP superfamily ID, and protein sequence. - 2018, The Author(s).