School Of Basic And Applied Sciences

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  • Item
    Transcriptome Analysis Revealed Behavior Complexity of Senescence Responses in Himalayan Tree Species Ulmus wallichiana
    (Springer, 2023-05-17T00:00:00) Singh, Amandeep; Majeed, Aasim; Sharma, Vikas; Gadri, Hari Shankar; Chowdhary, Md. Asif; Bhardwaj, Pankaj
    The Himalayas present a highly contrasting environment for the organisms that inhabit it, with summer and winter being the most disparate seasons. As winter approaches, the temperature drops, and deciduous plants, such as Ulmus wallichiana Planch., undergo senescence. In this study, we used the RNA-seq approach to generate a de novo transcriptome assembly of U. wallichiana, consisting of 300,067 unigenes. We investigated the differential expression pattern of these unigenes under changing climatic conditions, with a focus on the contrast between winter and summer. We observed that 2746 unigenes were differentially expressed, with a fold change of ? 2 and an FDR-adjusted p-value of ? 0.001. Among these unigenes, 37 were found to be related to senescence. We also identified nine DREB unigenes in the U. wallichiana transcriptome, which exhibited more or less contrasting patterns between summer and winter. These DREB unigenes may play an important role in regulating the plant�s tolerance to cold and/or drought stress. Our findings shed light on the complex and dynamic process of transcriptomic reprogramming that enables U. wallichiana to cope with seasonal changes. Furthermore, this study highlights the urgent need for further research on U. wallichiana, as this endangered plant species remains largely unexplored at the genomic level. � 2023, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
  • Item
    Microbial-based inoculants in sustainable agriculture: Current perspectives and future prospects
    (Elsevier, 2021-03-26T00:00:00) Dukare, Ajinath; Paul, Sangeeta; Kumar, Roshan; Sharma, Vikas
    The incessant use of agrochemicals for improved soil fertility and plant productivity has caused negative environmental impacts, including contamination of soil, groundwater, and aquifers. Hence, eco-friendly methods of plant and soil nutrient management are required to maintain sustained crop productivity and ecological stability. Beneficial microbial inoculants are potential elements of such management approaches. With the latest studies, this chapter summarizes the efforts in using beneficial microbes including plant growth-promoting rhizobacteria and mycorrhizal fungi for improving plant growth and its nutritional quality in a sustained manner. Studies with many microbial inoculants have demonstrated their beneficial role in plant growth through effective root colonization and induction of plant growth support mechanisms. Direct plant growth support by root associated microbes is mediated through enhanced nutrient acquisition and hormonal activation. Numerous antifungal activities of microbes, associated with pathogen inhibition, are usually linked to providing superior plant growth. Some inoculants have been found useful in abiotic stress alleviation and nutritional fortification of edible crops. The use of modern scientific advances for improving performances of microbial inoculants under stressed agriculture is also gaining scientific attention. Overall, the exploitation of beneficial microorganisms and their useful interactions with plants offer promising and eco-friendly strategies in the development of organic agriculture globally. � 2021 Elsevier Inc. All rights reserved.
  • Item
    Transcriptome Analysis Revealed Behavior Complexity of Senescence Responses in Himalayan Tree Species Ulmus wallichiana
    (Springer, 2023-05-17T00:00:00) Singh, Amandeep; Majeed, Aasim; Sharma, Vikas; Gadri, Hari Shankar; Chowdhary, Md. Asif; Bhardwaj, Pankaj
    The Himalayas present a highly contrasting environment for the organisms that inhabit it, with summer and winter being the most disparate seasons. As winter approaches, the temperature drops, and deciduous plants, such as Ulmus wallichiana Planch., undergo senescence. In this study, we used the RNA-seq approach to generate a de novo transcriptome assembly of U. wallichiana, consisting of 300,067 unigenes. We investigated the differential expression pattern of these unigenes under changing climatic conditions, with a focus on the contrast between winter and summer. We observed that 2746 unigenes were differentially expressed, with a fold change of ? 2 and an FDR-adjusted p-value of ? 0.001. Among these unigenes, 37 were found to be related to senescence. We also identified nine DREB unigenes in the U. wallichiana transcriptome, which exhibited more or less contrasting patterns between summer and winter. These DREB unigenes may play an important role in regulating the plant�s tolerance to cold and/or drought stress. Our findings shed light on the complex and dynamic process of transcriptomic reprogramming that enables U. wallichiana to cope with seasonal changes. Furthermore, this study highlights the urgent need for further research on U. wallichiana, as this endangered plant species remains largely unexplored at the genomic level. � 2023, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
  • Item
    Microbial-based inoculants in sustainable agriculture: Current perspectives and future prospects
    (Elsevier, 2021-03-26T00:00:00) Dukare, Ajinath; Paul, Sangeeta; Kumar, Roshan; Sharma, Vikas
    The incessant use of agrochemicals for improved soil fertility and plant productivity has caused negative environmental impacts, including contamination of soil, groundwater, and aquifers. Hence, eco-friendly methods of plant and soil nutrient management are required to maintain sustained crop productivity and ecological stability. Beneficial microbial inoculants are potential elements of such management approaches. With the latest studies, this chapter summarizes the efforts in using beneficial microbes including plant growth-promoting rhizobacteria and mycorrhizal fungi for improving plant growth and its nutritional quality in a sustained manner. Studies with many microbial inoculants have demonstrated their beneficial role in plant growth through effective root colonization and induction of plant growth support mechanisms. Direct plant growth support by root associated microbes is mediated through enhanced nutrient acquisition and hormonal activation. Numerous antifungal activities of microbes, associated with pathogen inhibition, are usually linked to providing superior plant growth. Some inoculants have been found useful in abiotic stress alleviation and nutritional fortification of edible crops. The use of modern scientific advances for improving performances of microbial inoculants under stressed agriculture is also gaining scientific attention. Overall, the exploitation of beneficial microorganisms and their useful interactions with plants offer promising and eco-friendly strategies in the development of organic agriculture globally. � 2021 Elsevier Inc. All rights reserved.