School Of Basic And Applied Sciences

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Author: search.filters.author.Singh, Amit

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    Colistin Resistance and Management of Drug Resistant Infections
    (Hindawi Limited, 2022-12-10T00:00:00) Sharma, Juhi; Sharma, Divakar; Singh, Amit; Sunita, Kumari
    Colistin resistance is a globalized sensible issue because it has been considered a drug of the last-line resort to treat drug-resistant bacterial infections. The product of the mobilized colistin resistance (mcr) gene and its variants are the significant causes of colistin resistance, which is emerging due to the frequent colistin use in veterinary, and these genes circulate among the bacterial community. Apart from mcr genes, some other intrinsic genes and proteins are also involved in colistin resistance. Researchers focus on the most advanced genomics (whole genome sequencing), proteomics, and bioinformatics approaches to explore the question of colistin resistance. To combat colistin resistance, researchers developed various strategies such as the development of newer drugs, the repurposing of existing drugs, combinatorial treatment by colistin with other drugs, a nano-based approach, photodynamic therapy, a CRISPRi-based strategy, and a phage-based strategy. In this timeline review, we have discussed the development of colistin resistance and its management in developing countries. � 2022 Juhi Sharma et al.
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    An Overview of Immunosensors and Their Application
    (Springer Nature, 2023-03-01T00:00:00) Gupta, Anil Kumar; Animesh, Sambhavi; Singh, Amit
    A key challenge in clinical healthcare is meeting the need to detect a disease at an early stage. Early and accurate diagnosis not only cuts the treatment cost but can also reduce disease burden, mortality rate, and social inequalities. Therefore, researchers are always searching for a method that allows rapid, simple, sensitive, selective, and cost-effective detection of the target biomarker (peptides, proteins, or nucleic acid). Immunosensors are one such point-of-care diagnostic device that can play an important role in almost all clinical healthcare fields. They are a promising alternative to the traditional immunoassays and state-of-the-art affinity sensors to diagnose clinically important analytes/antigens due to their high affinity, versatility, compact size, fast response time, minimum sample processing, and the measurements� reproducibility. For many decades now, significant advancement has been made in the immunosensor field in which the use of nanomaterials for increased sensitivity, multiplexing, or microfluidic-based devices may have the potential for promising use in clinical analysis. This chapter will provide an overview of the currently available immunosensor technology, its types that are currently being developed, and the limitations and future directions of immunosensor technology for the clinical laboratory. � The Editor (s) (if applicable) and the Author (s), under exclusive license to Springer Nature Singapore Pte Ltd.2023.
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    An Overview of Immunosensors and Their Application
    (Springer Nature, 2023-03-01T00:00:00) Gupta, Anil Kumar; Animesh, Sambhavi; Singh, Amit
    A key challenge in clinical healthcare is meeting the need to detect a disease at an early stage. Early and accurate diagnosis not only cuts the treatment cost but can also reduce disease burden, mortality rate, and social inequalities. Therefore, researchers are always searching for a method that allows rapid, simple, sensitive, selective, and cost-effective detection of the target biomarker (peptides, proteins, or nucleic acid). Immunosensors are one such point-of-care diagnostic device that can play an important role in almost all clinical healthcare fields. They are a promising alternative to the traditional immunoassays and state-of-the-art affinity sensors to diagnose clinically important analytes/antigens due to their high affinity, versatility, compact size, fast response time, minimum sample processing, and the measurements� reproducibility. For many decades now, significant advancement has been made in the immunosensor field in which the use of nanomaterials for increased sensitivity, multiplexing, or microfluidic-based devices may have the potential for promising use in clinical analysis. This chapter will provide an overview of the currently available immunosensor technology, its types that are currently being developed, and the limitations and future directions of immunosensor technology for the clinical laboratory. � The Editor (s) (if applicable) and the Author (s), under exclusive license to Springer Nature Singapore Pte Ltd.2023.
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    Colistin Resistance and Management of Drug Resistant Infections
    (Hindawi Limited, 2022-12-10T00:00:00) Sharma, Juhi; Sharma, Divakar; Singh, Amit; Sunita, Kumari
    Colistin resistance is a globalized sensible issue because it has been considered a drug of the last-line resort to treat drug-resistant bacterial infections. The product of the mobilized colistin resistance (mcr) gene and its variants are the significant causes of colistin resistance, which is emerging due to the frequent colistin use in veterinary, and these genes circulate among the bacterial community. Apart from mcr genes, some other intrinsic genes and proteins are also involved in colistin resistance. Researchers focus on the most advanced genomics (whole genome sequencing), proteomics, and bioinformatics approaches to explore the question of colistin resistance. To combat colistin resistance, researchers developed various strategies such as the development of newer drugs, the repurposing of existing drugs, combinatorial treatment by colistin with other drugs, a nano-based approach, photodynamic therapy, a CRISPRi-based strategy, and a phage-based strategy. In this timeline review, we have discussed the development of colistin resistance and its management in developing countries. � 2022 Juhi Sharma et al.
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    Bulbine frutescens phytochemicals as novel ABC-transporter inhibitor: A molecular docking and molecular dynamics simulation study
    (OAE Publishing Inc., 2021-01-08T00:00:00) Kushwaha, Prem Prakash; Maurya, Santosh Kumar; Singh, Amit; Prajapati, Kumari Sunita; Singh, Atul Kumar; Shuaib, Mohd; Kumar, Shashank
    Aim: The present in silico study aimed to evaluate the ATP-binding cassette (ABC) transporter inhibition potential of Bulbine frutescens (B. frutescens) phytochemicals. Methods: Several previous studies and databases were used to retrieve the ligands and target protein structure. The molecular docking study was performed using the Auto Dock Tools, and the GROMACS package was applied to accomplish molecular dynamics simulation. Results: Utilizing the molecular docking and simulation approach, ?25 phytochemicals were screened against the ABC transporter protein. Docking score analysis revealed that B. frutescens phytochemical 4?-Demethylknipholone 2?-?-D-glucopyranoside exhibited strong binding on the ABC transporter protein with a minimum binding score -9.8 kcal/mol in comparison to the standard ABC transporter inhibitor diltiazem (-6.86 kcal/mol). Furthermore, molecular dynamics simulation for 4?-Demethylknipholone 2?-?-D-glucopyranoside showed an acceptable root mean square deviation, radius of gyration, root mean square fluctuation, and hydrogen bond, in addition to other lead compounds. Conclusion: The in-silico study demonstrated that B. frutescens phytochemical 4?-Demethylknipholone 2?-?-D-glucopyranoside possesses anti-drug resistance properties and requires further testing in preclinical settings. � 2021 The Author(s).