School Of Basic And Applied Sciences

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Author: search.filters.author.Singh, KashmirHas files: No

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    Genome-wide identification of NAC transcription factors in Avena sativa under salinity stress
    (Elsevier B.V., 2023-10-29T00:00:00) Bokolia, Muskan; Singh, Baljinder; Kumar, Avneesh; Goyal, Nandni; Singh, Kashmir; Chhabra, Ravindresh
    Background: NAC (NAM, ATAF1/2, and CUC2) is one of the most prominent family of plant-specific transcription factors that play diverse roles in plant growth and development as well as in abiotic stress responses in plants. The members of this family are recognized by presence of typical conserved NAC domain at the N-terminal and diverse C-terminal region. Results: In this study, we have identified 101 Avena sativa NAC (AsNAC) genes from the available Avena genome database. Genes were analyzed for their physicochemical properties, conserved motifs, gene structure, chromosomal localization, phylogenetic relationship, and cis-acting elements. The phylogenetic analysis illustrated that there were 15 subgroups in both Avena sativa and Arabidopsis thaliana. Mainly four types of cis-acting regulatory elements were present in the promoter regions of NAC genes, including hormone-responsive, light-responsive, stress-responsive and growth and developmental responsive elements. The chromosomal mapping analysis concluded that 101 NAC genes of Avena sativa were unevenly distributed on 21 chromosomes. Expression analysis identified 27 Avena NAC genes that respond to salt stress based on transcriptomic data analysis available on the NCBI SRA database. Significance: The genome-wide identification and molecular analysis of NAC TFs involved in environmental stress responses have the ability to overcome the limitations that came across in producing the transgenic crops with superior quality and improved production under abiotic stressed conditions. Future prospectives: These NAC genes may be considered as potential candidates for further explorations of functional analysis and could be used to develop stress tolerant lines in Avena sativa. � 2023 The Author(s)
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    MSMEG-5850, a stress-induced TetR protein, involved in global transcription regulation in Mycobacterium smegmatis
    (Newlands Press Ltd, 2023-06-07T00:00:00) Singh, Parul; Kumar, Arbind; Chhabra, Ravindresh; Singh, Kashmir; Kaur, Jagdeep
    Aim: To decipher the role of MSMEG-5850 in the physiology of mycobacteria. Methods: MSMEG-5850 was knocked out and RNA sequencing was performed. MSMEG-5850 protein was purified from the Escherichia coli pET28a system. Electrophoretic mobility shift assay and size exclusion chromatography were used to determine the binding of MSMEG-5850 to its motif and binding stoichiometry. The effect of nutritional stress was monitored. Results: Transcriptome analysis revealed the differential expression of 148 genes in an MSMEG-5850 knockout strain. MSMEG-5850 had control over 50 genes because those genes had a binding motif upstream of their sequence. The electrophoretic mobility shift assay showed MSMEG-5850 bound to its motif as a monomer. MSMEG-5850 was upregulated under nutritional stress and promoted the survival of mycobacteria. Conclusion: The study confirms the role of MSMEG-5850 in global transcriptional regulation. � 2023 Future Medicine Ltd.