School Of Basic And Applied Sciences

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Author: search.filters.author.Singh, PriyankaHas files: Yes

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    Single-molecule force-unfolding of titin I27 reveals a correlation between the size of the surrounding anions and its mechanical stability
    (Royal Societty of chemistry, 2018) Muddassir, Mohd; Manna, Bharat; Singh, Priyanka; Singh, Surjeet; Kumar,Rajesh; Ghosh, Amit; Sharma, Deepak
    Each cellular protein is surrounded by a biochemical milieu that affects its stability and the associated function. The role of this surrounding milieu in the proteins’ mechanical stability remains largely unexplored. Herein, we report an as yet unknown correlation between the size of the surrounding anions and the mechanical stability of a protein. Using single-molecule force spectroscopy of the 27th domain (I27) of human cardiac muscle protein titin, we show that the average unfolding force of the protein decreases with increase in the ionic radii of the surrounding anions in the order Cl− > Br− > NO3− > I− > SO42− ≈ ClO4−, indicating an inverse correlation between anion size and the mechanical stability of I27. The destabilizing effect was attributed to the combined effect of increase in the unfolding rate constant and unfolding distance upon incubation with the anion. These findings reveal that anion size can significantly affect the mechanical resistance of proteins and thus could be a convenient and promising tool for regulating the mechanical stability of proteins.
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    Downregulation of Bcr-Abl oncogene in Chronic Myeloid Leukemia (CML) by microRNAs in case of Imatinib resistance
    (ASIAN PACIFIC JOURNAL OF HEALTH SCIENCES, 2018) Singh, Priyanka; Gupta, Sonu Kumar; Ali, Vilayat; Verma, Malkhey
    The well-known myeloproliferative malignancy, chronic myeloid leukemia (CML), causes due to the formation of short and modified Philadelphia chromosome having the Bcr-Abl oncogene. Many therapeutic approaches have been made for the treatment of CML, the best one was the development of Tyrosine Kinase Inhibitors (TKIs), mainly Imatinib. But after the development of mutation against Imatinib, researchers moved towards RNA interference (RNAi) of BCR-ABL mRNA via microRNAs. In this review, we identified 105 miRNAs by Target Scan, miRbase and miRNAMap, which target the proteins of CML signaling pathway. These are selected on the basis of their constitutive activation in the Bcr-Abl positive cell lines. Targeting these proteins by miRNAs might effectively enhance chemotherapy-induced cytotoxicity in CML cells. Out of these 105 miRNAs, 21 were found to commonly effective against those proteins. These 21 microRNAs may or may not have been studied in CML cases, but have been studied in other solid or myeloid tumors. This review might be helpful in extending the studies regarding regulation of CML signaling proteins by miRNAs.
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    Drought and heat tolerance in chickpea: Transcriptome and morphophysiological changes under individual and combined stress
    (Springer India, 2017) Yadav, Renu; Juneja, Sumandeep; Singh, Priyanka; Kumar, Sanjeev
    Increase in global temperature due to climate change is the major concern and known to have detrimental effect on many agricultural crops. Chickpea (Cicer arietinum L.) is an important legume grown in the arid and semiarid region of the world. Chickpea being a heat sensitive crop is greatly affected by heat stress during both vegetative and reproductive stages. Stress resistance mechanism of chickpea involves signal perception, transduction, and subsequent activation of stress-responsive genes encoding reactive oxygen species (ROS) scavenging and osmolyte, chaperones, and aquaporins. There are different stress perception and signaling pathways, both in drought and high-temperature stress, but some common pathways also exist between the two mechanisms. The present chapter summarizes the cross talk between the drought and heat stress and the molecular mechanism underlying individual stress. Field plants are exposed to multiple stresses, and the combined effect might be antagonistic or synergistic. Hence, improving stress tolerance of plants requires a reevaluation, taking into account the effect of multiple stresses on plant metabolism and stress resistance. ? Springer (India) Pvt. Ltd. 2017. All rights reserved.