School Of Basic And Applied Sciences

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    Pseudomonas citronellolis; a multi-metal resistant and potential plant growth promoter against arsenic (V) stress in chickpea
    (Elsevier, 2019) Adhikary, Arindam; Kumar, R; Pandir,R; Bhardwaj, Pankaj; Wusirika, Ramakrishna; Kumar, Sanjeev
    Soil micro-biota plays a vital role in maintaining plant growth and fitness under normal and adverse conditions. Pseudomonas is one of the most important free-living and copious genera in south-west Punjab and involved in plant growth promotion under heavy metal stress. In this study, we have studied microbial diversity of the agricultural and marginal land based on 16S rRNA gene and screened eight strains of Pseudomonas for its tolerances towards various heavy metals and for plant growth promoting properties (PGP). The best strain is tested in chickpea plants against Arsenic (As5+) stress. All the strains responded differently to heavy metals viz. Arsenic, (As5+ (0.3–0.5M) and As3+ (250 μg mL−1) Cadmium (Cd2+) (250–350 μg mL−1), Chromium (Cr2+) (200–350 μg mL−1) and Mercury (Hg2+) (1–2 μg mL−1). Out of eight strains, only two strains (KM594398 and KM594397) showed plant growth promoting characters, concurrently they were highly tolerant to Arsenic (As5+). Pseudomonas citronellolis (PC) (KM594397) showed the best results in terms of As5+ tolerance and plant growth promoting activity, hence further tested for actual plant growth response in chickpea (Cicer arietinum L.) under As5+ (10–160 mg kg−1) stress. Pseudomonas citronellolis enhanced plant growth and dry biomass under As5+ stress. High As5+ tolerance and plant growth promoting activity of Pseudomonas citronellolis in chickpea especially designate this strain suitable for marginal lands and heavy metals contaminated sites. © 2019 Elsevier Masson SAS
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    Phytogenetic analysis of bacterial diversity of heavy metal affected
    (Central University of Punjab, 2012) Kumar, Rajeev; Kumar, Sanjeev
    The soil of Bathinda region is affected by heavy metals due to anthropogenic activity, industrial effluent and fly ash. Three soil samples in triplicate were collected from three different places of Bathinda region, abandoned soil, agricultural soil, thermal power plant fly ash soil. Based on 16S rRNA gene cloning and sequence analysis, phylogentic analysis of bacterial diversity was performed. 366 clones were picked and through colony PCR 128 positive clones were chosen. Out of 128 clones, 126 clones were sequenced in three fragments and all three fragments aligned using SeqMan Lasergene ver.10.0 (DNA STAR) software. BLAST analysis of sequenced and aligned samples was carried out at NCBI to find out the homology with different groups of bacteria. All 126 clones aligned for phylogenetic analysis by MEGA 5 (Neighbor Joining method). 32 genus belong to 8 phyla were observed in all three samples. Most dominant phylum is Proteobacteria followed by Bacteriodates. Pseudomonas spp. is dominant in agricultural and thermal power plant soil. Abandoned soil showed maximum diversity followed by thermal power plant soil. In agricultural soil minimum diversity was observed. So with this investigation it can be concluded that lands without anthropogenic activities are rich in bacterial diversity as compared to more anthropogenic active areas.