School Of Basic And Applied Sciences

Permanent URI for this communityhttps://kr.cup.edu.in/handle/32116/17

Browse

Filters

2012 - 20193

Settings

Subject: search.filters.subject.Cicer arietinum

Search Results

Now showing 1 - 3 of 3
  • No Thumbnail Available
    Item
    Genome-wide identification, characterization and in-silico profiling of genes encoding FAD (fatty acid desaturase) proteins in chickpea (Cicer arietinum L.)
    (Elsevier, 2019) Saini, Rashmi; Kumar, Sanjeev
    Fatty acid desaturases (FADs) are involved in introducing double bonds into the hydrocarbon chains of fatty acids leading to the formation of unsaturated fatty acids. These unsaturated fatty acids are known to play important role in plant development and generating stress tolerance against various environmental stresses. In this study, 21 full-length FAD genes were identified in Desi chickpea (Cicer arietinum L.) and 18 FAD genes in Kabuli chickpea genome by a thorough annotation exercise. An extensive analysis was done to establish their chromosomal locations, construct a phylogeny, structure prediction, motif analysis, in-silico expression analysis and co-expression with other interconnected pathways. In desi chickpea, out of 21 FAD genes, 19 were located on seven chromosomes and two additional scaffolds whereas in Kabuli, out of 18 FAD genes, 17 were located on seven chromosomes and an additional scaffold. Identified soluble and membrane-bound CaFADs were phylogenetically clustered into four and eight subfamilies, respectively with their counterparts from other plants. It was observed that gene structures, motif composition and positions were highly conserved in each subfamily. Furthermore, in-silico gene expression analysis of CaFAD genes in various tissues as well as under abiotic stress conditions revealed differential expression patterns in different tissues and developmental stages. Nine FAD genes were down-regulated in all three abiotic stresses as compared to control. Whereas, five FAD genes were up-regulated in shoot tissue during drought, two FAD genes during salinity and three FAD genes were up-regulated during cold stress. In root tissue, 9 FAD genes were down-regulated and 3 FAD genes were up-regulated during all three abiotic stresses. In co-expression analysis, fatty acid synthesis, TAG synthesis genes and Jasmonic acid (JA) biosynthesis genes were expressed more during flower development and shoot apical meristem. JA signaling gene (CaJAR1) was also expressed more in bud, shoot apical meristem and seeds. No other relationship was observed with respect to modulation during abiotic stresses.
  • Thumbnail Image
    Item
    Global transcriptome and coexpression network analyses reveal cultivar-specific molecular signatures associated with seed development and seed size/weight determination in chickpea.
    (Wiley, 2017) Garg, Rohini; Singh, Vikash K.; Rajkumar, Mohan Singh; Kumar, Vinay; Jain, Mukesh
    Seed development is an intricate process regulated via a complex transcriptional regulatory network. To understand the molecular mechanisms governing seed development and seed size/weight in chickpea, we performed a comprehensive analysis of transcriptome dynamics during seed development in two cultivars with contrasting seed size/weight (small-seeded, Himchana 1 and large-seeded, JGK 3). Our analysis identified stage-specific expression for a significant proportion (>13%) of the genes in each cultivar. About one half of the total genes exhibited significant differential expression in JGK 3 as compared with Himchana 1. We found that different seed development stages can be delineated by modules of coexpressed genes. A comparative analysis revealed differential developmental stage specificity of some modules between the two cultivars. Furthermore, we constructed transcriptional regulatory networks and identified key components determining seed size/weight. The results suggested that extended period of cell division during embryogenesis and higher level of endoreduplication along with more accumulation of storage compounds during maturation determine large seed size/weight. Further, we identified quantitative trait loci-associated candidate genes harboring single nucleotide polymorphisms in the promoter sequences that differentiate small- and large-seeded chickpea cultivars. The results provide a valuable resource to dissect the role of candidate genes governing seed development and seed size/weight in chickpea.
  • Thumbnail Image
    Item
    Temperature-stratified screening of chickpea (Cicer arietinum L.) genetic resource collections reveals very limited reproductive chilling tolerance compared to its annual wild relatives
    (2012) Berger, J.D.; Kumar, Sanjeev; Nayyar, H.; Street, K. A.; Sandhu, J. S.; Henzell, J. M.; Kaur, J.; Clark, H.C.
    Low reproductive chilling tolerance in chickpea impairs ovule fertilization, delaying pod set, exposing the crop to terminal drought throughout much of its distribution range. Despite this realization, little progress has been made because of the limited genetic variation available to breeders. To address this issue a wide range of domesticated (n= 1762) and wild Cicer (n= 200) germplasm collected from sites stratified by flowering phase temperature was extensively field evaluated, and compared with Lupinus angustifolius, a well-adapted Mediterranean winter annual. Chilling tolerance was estimated by regressing the time interval between pod set and first flower against mean post-anthesis temperature. Field screening was augmented by smaller scale experiments evaluating the effects of contrasting post-anthesis temperature regimes on plant growth and productivity, pollen function and subsequent pod set in temperature-controlled cabinets.Chickpea was less chilling tolerant than its wild relatives, the flower-pod interval increasing curvi-linearly as sites became cooler, with a strong effects between 11 and 16. ?C, tailing off after 17.5. ?C, but remaining statistically significant. There is little useful variation for chilling tolerance within domesticated chickpea. Small, albeit statistically significant differences in pod set delay in chickpea collected from contrasting flowering phase habitats, were marginal compared to more tolerant species such as Cicer bijugum, Cicer judaicum and L. angustifolius, and to a lesser extent Cicer reticulatum, Cicer pinnatifidum, and Cicer echinospermum. No differences were observed between desi and kabuli types. Field screening identified robust chilling tolerance in a C. echinospermum accession that commenced podding earlier, at lower temperatures (10.0. ?C), and yielded 5 times more than Rupali, the most productive chickpea. Controlled temperature experiments confirmed that in contrast to chickpea, pollen germination, viability, frequency on the stigma surface and subsequent pod set were unaffected by low post-anthesis temperatures (13/7. ?C) in C. echinospermum and L. angustifolius. Our results indicate that chickpea is even more chilling sensitive than previously thought. Because C. echinospermum is inter-fertile with chickpea, it has considerable potential both as a donor of robust chilling tolerance and as an agent for investigating resistance mechanisms. ? 2011.